Abstract
Our studies of SecM (secretion monitor) in E. coli have revealed that some amino acid sequences can interact with ribosomal interior components, particularly with gate components of the exit tunnel, thereby interfering with their own translation elongation. Such translation arrest can be regulated by interaction of the N-terminal portion of the nascent polypeptide with other cellular components outside the ribosome. These properties of nascent proteins can in turn provide regulatory mechanisms by which the expression of genetic information at different levels is regulated.
MeSH terms
- Adenosine Triphosphatases / biosynthesis
- Amino Acid Sequence
- Amino Acids / chemistry
- Bacterial Proteins / biosynthesis
- Gene Expression Regulation / genetics*
- Gene Expression Regulation / physiology*
- Membrane Transport Proteins / biosynthesis
- Molecular Sequence Data
- Peptide Chain Elongation, Translational
- Protein Biosynthesis*
- Protein Transport
- Proteins / metabolism*
- Ribosomes / metabolism*
- SEC Translocation Channels
- SecA Proteins
Substances
- Amino Acids
- Bacterial Proteins
- Membrane Transport Proteins
- Proteins
- SEC Translocation Channels
- Adenosine Triphosphatases
- SecA Proteins