Role of surface charge and oxidative stress in cytotoxicity and genotoxicity of graphene oxide towards human lung fibroblast cells
Anxin Wang
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101 China
University of the Chinese Academy of Sciences, Beijing, 100049 China
These authors contributed equally to this work.Search for more papers by this authorKefeng Pu
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101 China
University of the Chinese Academy of Sciences, Beijing, 100049 China
These authors contributed equally to this work.Search for more papers by this authorBing Dong
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101 China
University of the Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorYang Liu
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101 China
University of the Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorLiming Zhang
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
Search for more papers by this authorZhijun Zhang
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
Search for more papers by this authorWei Duan
School of Medicine, Deakin University, VIC, 3217 Australia
Search for more papers by this authorCorresponding Author
Yimin Zhu
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
Correspondence to: Yimin Zhu, 398 Ruoshui Road, Suzhou Industrial Park, Suzhou 215123, China. E-mail: [email protected]Search for more papers by this authorAnxin Wang
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101 China
University of the Chinese Academy of Sciences, Beijing, 100049 China
These authors contributed equally to this work.Search for more papers by this authorKefeng Pu
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101 China
University of the Chinese Academy of Sciences, Beijing, 100049 China
These authors contributed equally to this work.Search for more papers by this authorBing Dong
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101 China
University of the Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorYang Liu
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101 China
University of the Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorLiming Zhang
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
Search for more papers by this authorZhijun Zhang
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
Search for more papers by this authorWei Duan
School of Medicine, Deakin University, VIC, 3217 Australia
Search for more papers by this authorCorresponding Author
Yimin Zhu
Suzhou Key Laboratory of Nanobiomedicine, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123 China
Correspondence to: Yimin Zhu, 398 Ruoshui Road, Suzhou Industrial Park, Suzhou 215123, China. E-mail: [email protected]Search for more papers by this authorABSTRACT
Recently, attempts have been made to apply graphene oxide (GO) in the field of biology and medicine, such as DNA sensing and drug delivery with some necessary modifications. Therefore, the toxicity of GO must be evaluated before it is applied further in biomedicine. In this paper, the cytotoxicity and genotoxicity of GO to human lung fibroblast (HLF) cells have been assessed with methyl thiazolyl tetrazolium (MTT), sub-G1 measurement and comet assays, and the mechanism of its toxicity has been explored. Various modifications of GO have been made to help us determine the factors which could affect the toxicity of GO. The results indicated that cytotoxicity and genotoxicity of GO to HLF cells were concentration dependent, and the genotoxicity induced by GO was more severe than the cytotoxicity to HLF cells. Oxidative stress mediated by GO might explain the reason of its toxic effect. Furthermore, the electronic charge on the surface of GO would play a very important role in the toxicity of GO to HLF cells. Copyright © 2013 John Wiley & Sons, Ltd.
Supporting Information
Supporting information can be found in the online version of this article.
| Filename | Description |
|---|---|
| jat_2877_sm_Fig_S1.tifTIFF image, 8 MB | Supplementary Figure S1 |
| jat_2877_sm_Fig_S2.tifTIFF image, 443.4 KB | Supplementary Figure S2 |
| jat_2877_sm_table_1.docxWord 2007 document , 13.7 KB | Zeta potential distribution of graphene oxide (GO), lactobionic acid-polyethylene glycol (LA-PEG)-GO, PEG-GO and polyethylenimine (PEI)-GO |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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