MicroRNA-27a-3p relieves inflammation and neurologic impairment after cerebral ischaemia reperfusion via inhibiting lipopolysaccharide induced TNF factor and the TLR4/NF-κB pathway
Jing Luo
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorJunjie Li
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorLi Xiong
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorLinna Fan
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorLijia Peng
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorYuan Yang
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorCorresponding Author
Di Lu
Incubation Center for Scientific and Technological Achievements, Kunming Medical University, Kunming, Yunnan, China
Correspondence
Jianlin Shao, Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Wuhua District, Kunming, Yunnan 650000, China.
Email: [email protected]
Di Lu, Incubation Center for Scientific and Technological Achievements, Kunming Medical University, Kunming, Yunnan 650500, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jianlin Shao
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Correspondence
Jianlin Shao, Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Wuhua District, Kunming, Yunnan 650000, China.
Email: [email protected]
Di Lu, Incubation Center for Scientific and Technological Achievements, Kunming Medical University, Kunming, Yunnan 650500, China.
Email: [email protected]
Search for more papers by this authorJing Luo
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorJunjie Li
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorLi Xiong
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorLinna Fan
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorLijia Peng
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorYuan Yang
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Search for more papers by this authorCorresponding Author
Di Lu
Incubation Center for Scientific and Technological Achievements, Kunming Medical University, Kunming, Yunnan, China
Correspondence
Jianlin Shao, Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Wuhua District, Kunming, Yunnan 650000, China.
Email: [email protected]
Di Lu, Incubation Center for Scientific and Technological Achievements, Kunming Medical University, Kunming, Yunnan 650500, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jianlin Shao
Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China
Correspondence
Jianlin Shao, Department of Anesthesiology, First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Wuhua District, Kunming, Yunnan 650000, China.
Email: [email protected]
Di Lu, Incubation Center for Scientific and Technological Achievements, Kunming Medical University, Kunming, Yunnan 650500, China.
Email: [email protected]
Search for more papers by this authorJing Luo and Junjie Li contribute equally.
Edited by: Angeles Almeida
Funding information: Applied Basic Research Joint Project of Yunnan Province, Grant/Award Number: 2017FE468(-034); Weifeng Yu Expert Workstation project, Grant/Award Number: 2017IC067; Industry Technology Leading Talent Training Program in Yunnan, Grant/Award Number: YLXL20170054; Key Applied Basic Research Program of Yunnan Province, Grant/Award Number: 2018FA042; National Nature Science Foundation of China, Grant/Award Numbers: 81760248, 81960250
Abstract
Cerebral ischaemia reperfusion (CIR) affects microRNA (miR) expression and causes substantial inflammation. Here, we investigated the influence and underlying mechanism of miR-27a-3p in rats with CIR. First, biliverdin treatment relieved cerebral infarction and decreased the levels of serum interleukin (IL)-1β, IL-6, and TNF-α. Through our previous study, we found key miR-27a-3p and its targeted gene LITAF might involve in the molecular mechanism of CIR. Then, the regulation between miR-27a-3p and LITAF was verified by the temporal miR-27a-3p and LITAF expression profiles and luciferase assay. Moreover, intracerebroventricular injection of the miR-27a-3p mimic significantly decreased the LITAF, TLR4, NF-κB, and IL-6 levels at 24 h post-surgery, whereas miR-27a-3p inhibitor reversed these effects. Furthermore, miR-27a-3p mimic could relieve cerebral infarct and neurologic deficit after CIR. In addition, injection of miR-27a-3p mimic decreased neuronal damage induced by CIR. Taken together, our results suggest that miR-27a-3p protects against CIR by relieving inflammation, neuronal damage, and neurologic deficit via regulating LITAF and the TLR4/NF-κB pathway.
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
Open Research
PEER REVIEW
The peer review history for this article is available at https://publons.com/publon/10.1111/ejn.15720.
DATA AVAILABILITY STATEMENT
Data are available on request.
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