Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate

Biochem Biophys Res Commun. 2009 Aug 14;386(1):11-5. doi: 10.1016/j.bbrc.2009.05.117. Epub 2009 May 30.

Abstract

By its antioxidant effect, molecular hydrogen gas (H2) was reported to protect organs from tissue damage induced by ischemia reperfusion. To evaluate its anti-inflammatory effects, we established a mouse model of human inflammatory bowel disease (IBD) by supplying mice with water containing (1) dextran sodium sulfate (DSS) (5%), (2) DSS (5%) and H2, or (3) H2 only ad libitum up to 7 days. At day-7, DSS-induced pathogenic outcomes including, loss of body weight, increase of colitis score, pathogenic shortening of colon length, elevated level of IL-12, TNF-alpha and IL-1beta in colon lesion, were significantly suppressed by the addition of H2 to DSS solution. Histological analysis also revealed that the DSS-mediated colonic tissue destruction accompanied by macrophage infiltration was remarkably suppressed by H2. Therefore, the present study indicated that H2 can prevent the development of DSS-induced colitis in mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Inhalation
  • Animals
  • Antioxidants / administration & dosage*
  • Colitis / chemically induced
  • Colitis / drug therapy*
  • Colitis / immunology
  • Colitis / pathology
  • Colon / drug effects*
  • Colon / immunology
  • Colon / pathology
  • Cytokines / biosynthesis
  • Dextran Sulfate / toxicity
  • Disease Models, Animal
  • Down-Regulation
  • Humans
  • Hydrogen / administration & dosage*
  • Macrophages / drug effects
  • Macrophages / immunology
  • Male
  • Mice
  • Mice, Inbred BALB C

Substances

  • Antioxidants
  • Cytokines
  • Hydrogen
  • Dextran Sulfate