Abstract
Studies suggest an association between consumption of a high-cholesterol diet and periodontitis. We addressed the mechanism by which high dietary cholesterol could be detrimental to periodontal health in a rat model. Feeding a high-cholesterol diet augmented the effects of bacterial pathogens and their products (e.g., lipopolysaccharide and proteases) on production of pro-inflammatory cytokines in fibroblasts. High dietary cholesterol also increased mitochondrial 8-hydroxydeoxyguanosine in the periodontal tissues. These results suggest that excessive tissue oxidative damage induced by high dietary cholesterol could potentiate pro-inflammatory cytokine production by fibroblasts stimulated with bacterial pathogens.
Publication types
- Research Support, Non-U.S. Gov't
MeSH terms
- 8-Hydroxy-2'-Deoxyguanosine
- Animal Feed
- Animals
- Cholesterol / administration & dosage*
- Cholesterol / blood
- Cholesterol / pharmacology*
- Deoxyguanosine / analogs & derivatives
- Deoxyguanosine / metabolism
- Disease Models, Animal
- Fibroblasts
- Interleukin-1 / metabolism
- Male
- Mitochondria / metabolism
- Oxidation-Reduction / drug effects
- Periodontitis / chemically induced
- Periodontitis / metabolism*
- Periodontitis / microbiology
- Periodontitis / pathology*
- Rats
- Rats, Wistar
- Triglycerides / blood
- Tumor Necrosis Factor-alpha / metabolism
Substances
- Interleukin-1
- Triglycerides
- Tumor Necrosis Factor-alpha
- 8-Hydroxy-2'-Deoxyguanosine
- Cholesterol
- Deoxyguanosine