Abstract
Oxidative stress is associated with age-related reactions. The anti-oxidative effects of a reduced form of co-enzyme Q10 (rCoQ10) suppress oxidative stress, which may contribute to the prevention of age-related inflammatory reactions. We examined the effects of topically applied rCoQ10 on periodontal inflammatory reactions in a rat aging model. Male Fischer 344 rats, 2 (n = 6) and 4 mos (n = 18) of age, were used. All of the two-month-old rats and 6 of the four-month-old rats were sacrificed and 12 remaining four-month-old rats received topically applied ointment with or without 1% rCoQ10 on the gingival surface until they reached 6 mos of age. The rats showed an age-dependent increase in circulating oxidative stress. RCoQ10 decreased oxidative DNA damage and tartrate-resistant acid-phosphatase-positive osteoclasts in the periodontal tissue at 6 mos of age as compared with the control. The same conditions lowered gene expression of caspase-1 and interleukin-1β in the periodontal tissue. Furthermore, Nod-like receptor protein 3 inflammasomes were less activated in periodontal tissues from rCoQ10-treated rats as compared with the control rats. Our results suggest that rCoQ10 suppresses age-related inflammatory reactions and osteoclast differentiation by inhibiting oxidative stress.
Keywords: aging; coenzyme Q10; inflammasomes; oxidative stress; periodontium; preclinical drug evaluation.
Publication types
- Comparative Study
- Research Support, Non-U.S. Gov't
MeSH terms
- 8-Hydroxy-2'-Deoxyguanosine
- Acid Phosphatase / analysis
- Actins / drug effects
- Age Factors
- Aging / drug effects*
- Alveolar Process / drug effects
- Alveolar Process / pathology
- Animals
- Antioxidants / pharmacology
- Apoptosis Regulatory Proteins
- CARD Signaling Adaptor Proteins
- Carrier Proteins
- Caspase 1 / drug effects
- Cytoskeletal Proteins / drug effects
- DNA Damage / drug effects
- Deoxyguanosine / analogs & derivatives
- Deoxyguanosine / analysis
- Deoxyguanosine / blood
- Electron Transport Chain Complex Proteins / pharmacology*
- Gingiva / drug effects
- Gingiva / pathology
- Inflammasomes / drug effects
- Interleukin-1beta / drug effects
- Isoenzymes / analysis
- Male
- Models, Animal
- NF-kappa B / drug effects
- NLR Family, Pyrin Domain-Containing 3 Protein
- Osteoclasts / drug effects
- Oxidative Stress / drug effects
- Periodontium / drug effects*
- Periodontium / pathology
- Random Allocation
- Rats
- Rats, Inbred F344
- Receptors, Cytoplasmic and Nuclear / drug effects
- Tartrate-Resistant Acid Phosphatase
- Ubiquinone / analogs & derivatives*
- Ubiquinone / blood
- Ubiquinone / pharmacology
- Vitamins / pharmacology*
Substances
- Actins
- Antioxidants
- Apoptosis Regulatory Proteins
- CARD Signaling Adaptor Proteins
- Carrier Proteins
- Cytoskeletal Proteins
- Electron Transport Chain Complex Proteins
- Inflammasomes
- Interleukin-1beta
- Isoenzymes
- NF-kappa B
- NLR Family, Pyrin Domain-Containing 3 Protein
- Nlrp3 protein, rat
- Pycard protein, rat
- Receptors, Cytoplasmic and Nuclear
- Vitamins
- Ubiquinone
- 8-Hydroxy-2'-Deoxyguanosine
- Acid Phosphatase
- Tartrate-Resistant Acid Phosphatase
- Caspase 1
- coenzyme Q10
- Deoxyguanosine