ACS Publications. Most Trusted. Most Cited. Most Read

OR SEARCH CITATIONS

My Activity
Publications
CONTENT TYPES

Figure 1Loading Img
Download Hi-Res ImageDownload to MS-PowerPointCite This:J. Agric. Food Chem. 1998, 46, 4, 1433-1436
RETURN TO ISSUEPREVArticleNEXT

Comparison of a Microbiological Assay and a Fully Automated Chemiluminescent System for the Determination of Vitamin B12 in Food

View Author Information
Department of Food and Nutrition, Kochi Women's University, Kochi 780, Japan, Laboratory of Nutrition and Food Science, Hagoromo-gakuen College, Sakai 592, Japan, and Department of Applied Biological Chemistry, Osaka Prefecture University, Sakai 593, Japan
Cite this: J. Agric. Food Chem. 1998, 46, 4, 1433–1436
Publication Date (Web):February 26, 1998
https://doi.org/10.1021/jf970807j
Copyright © 1998 American Chemical Society
Request reuse permissions

    Article Views

    794

    Altmetric

    -

    Citations

    44
    LEARN ABOUT THESE METRICS
    Other access options
    Get e-Alerts
    SUBJECTS:

    Abstract

    A fully automated chemiluminescence analyzer for the determination of vitamin B12 in serum has been commercialized and clinically used. To determine the applicability of this apparatus in food analysis, vitamin B12 was assayed in foods by the chemiluminescent method, which was compared with a microbiological method. In shellfishes and spirulina, the values determined by the microbiological method were ∼6−8-fold greater than the values determined by the chemiluminescence method, although there was good similarity between the values by the two methods in other foods. Except for the shellfishes and spirulina, which contained substantial amounts of vitamin B12-substitutive compounds or inactive vitamin B12 analogues (or both), the observed correlation coefficient between the methods in the foods tested was excellent (r = 0.99, y = 1.2x − 1.1, n = 9). The chemiluminescence method was suitable for the determination of vitamin B12 in foods as well as in serum and was simpler (fully automated) and more rapid (180 samples analyzed per hour), highly selective (use of intrinsic factor, the most specific vitamin B12-binding protein), and reproducible (coefficients of variation of 1.2−6.7%) than the microbiological method.

    Keywords: Vitamin B12; bioassay; chemiluminescence; intrinsic factor; food

    Read this article

    To access this article, please review the available access options below.

    Get instant access

    Purchase Access

    Read this article for 48 hours. Check out below using your ACS ID or as a guest.

    Recommended

    Access through Your Institution

    You may have access to this article through your institution.

    Your institution does not have access to this content. You can change your affiliated institution below.

    *

     Author to whom correspondence should be addressed (telephone and fax +81-888-31-2876; e-mail [email protected]).

    †

     Kochi Women's University.

    ‡

     Hagoromo-gakuen College.

    §

     Osaka Prefecture University.

    Cited By

    This article is cited by 44 publications.

    1. Anantharajappa Kumudha, Sagaya Selva Kumar, Munna Singh Thakur, Gokare Aswathanarayana Ravishankar, and Ravi Sarada . Purification, Identification, and Characterization of Methylcobalamin from Spirulina platensis. Journal of Agricultural and Food Chemistry 2010, 58 (18) , 9925-9930. https://doi.org/10.1021/jf102159j
    2. Emi Miyamoto,, Yuri Tanioka,, Tomoyuki Nakao,, Florin Barla,, Hiroshi Inui,, Tomoyuki Fujita,, Fumio Watanabe, and, Yoshihisa Nakano. Purification and Characterization of a Corrinoid-Compound in an Edible Cyanobacterium Aphanizomenon flos-aquae as a Nutritional Supplementary Food. Journal of Agricultural and Food Chemistry 2006, 54 (25) , 9604-9607. https://doi.org/10.1021/jf062300r
    3. Hiromi Kittaka-Katsura,, Syuhei Ebara,, Fumio Watanabe, and, Yoshihisa Nakano. Characterization of Corrinoid Compounds from a Japanese Black Tea (Batabata-cha) Fermented by Bacteria. Journal of Agricultural and Food Chemistry 2004, 52 (4) , 909-911. https://doi.org/10.1021/jf030585r
    4. Hiromi Kittaka-Katsura,, Tomoyuki Fujita,, Fumio Watanabe, and, Yoshihisa Nakano. Purification and Characterization of a Corrinoid Compound from Chlorella Tablets as an Algal Health Food. Journal of Agricultural and Food Chemistry 2002, 50 (17) , 4994-4997. https://doi.org/10.1021/jf020345w
    5. Fumio Watanabe,, Emi Miyamoto, and, Yoshihisa Nakano. Inactive Corrinoid-Compound Significantly Decreases in Spirulina platensis Grown in a Cobalt-Deficient Medium. Journal of Agricultural and Food Chemistry 2001, 49 (11) , 5685-5688. https://doi.org/10.1021/jf010733i
    6. Emi Miyamoto,, Fumio Watanabe,, Syuhei Ebara,, Shigeo Takenaka,, Hiroyuki Takenaka,, Yuji Yamaguchi,, Norihide Tanaka,, Hiroshi Inui, and, Yoshihisa Nakano. Characterization of a Vitamin B12 Compound from Unicellular Coccolithophorid Alga (Pleurochrysis carterae). Journal of Agricultural and Food Chemistry 2001, 49 (7) , 3486-3489. https://doi.org/10.1021/jf0101556
    7. Fumio Watanabe,, Hiromi Katsura,, Shigeo Takenaka,, Tomoyuki Fujita,, Katsuo Abe,, Yoshiyuki Tamura,, Toshiyuki Nakatsuka, and, Yoshihisa Nakano. Pseudovitamin B12 Is the Predominant Cobamide of an Algal Health Food, Spirulina Tablets. Journal of Agricultural and Food Chemistry 1999, 47 (11) , 4736-4741. https://doi.org/10.1021/jf990541b
    8. Fumio Watanabe,, Shigeo Takenaka,, Hiromi Katsura,, S. A. M. Zakir Hussain Masumder,, Katsuo Abe,, Yoshiyuki Tamura, and, Yoshihisa Nakano. Dried Green and Purple Lavers (Nori) Contain Substantial Amounts of Biologically Active Vitamin B12 but Less of Dietary Iodine Relative to Other Edible Seaweeds. Journal of Agricultural and Food Chemistry 1999, 47 (6) , 2341-2343. https://doi.org/10.1021/jf981065c
    9. Jueling Chen, Chunling Mao, Heng Ye, Xun Gao, Longshan Zhao. Natural biomass carbon Dots-Based fluorescence sensor for high precision detection of vitamin B12 in serum. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2024, 305 , 123459. https://doi.org/10.1016/j.saa.2023.123459
    10. Sergey N. Fedosov, Ebba Nexo, Christian W. Heegaard, Jarrod Goldin, Joel B. Mason. Protein binding assays for an accurate differentiation of vitamin B12 from its inactive analogue. A study on edible cricket powder. Food Chemistry: X 2023, 19 , 100824. https://doi.org/10.1016/j.fochx.2023.100824
    11. Milena Pieńkos, Beata Zadykowicz. Computational Insights on the Mechanism of the Chemiluminescence Reaction of New Group of Chemiluminogens—10-Methyl-9-thiophenoxycarbonylacridinium Cations. International Journal of Molecular Sciences 2020, 21 (12) , 4417. https://doi.org/10.3390/ijms21124417
    12. Yong Jia, Yue Hu, Yingping Li, Qi Zeng, Xiaohui Jiang, Zhengjun Cheng. Boron doped carbon dots as a multifunctional fluorescent probe for sorbate and vitamin B12. Microchimica Acta 2019, 186 (2) https://doi.org/10.1007/s00604-018-3196-5
    13. Beom-Gyun Jeong, Geumju Mun, Jiyoung Jung, Jiyeon Chun. Immunoaffinity-HPLC Analysis for Vitamin B12 of Korean Cattle-Hanwoo (Bos taurus coreanae). Food Analytical Methods 2018, 11 (9) , 2597-2606. https://doi.org/10.1007/s12161-018-1248-4
    14. Daphna K Dror, Lindsay H Allen. Vitamin B-12 in Human Milk: A Systematic Review. Advances in Nutrition 2018, 9 , 358S-366S. https://doi.org/10.1093/advances/nmx019
    15. Beata Zadykowicz, Piotr Storoniak. Lattice energetics and thermochemistry of acridine derivatives and substituted acridinium trifluoromethanesulphonates. Journal of Thermal Analysis and Calorimetry 2017, 129 (3) , 1613-1624. https://doi.org/10.1007/s10973-017-6306-4
    16. Justyna Czechowska, Alicja Kawecka, Anna Romanowska, Maria Marczak, Paweł Wityk, Karol Krzymiński, Beata Zadykowicz. Chemiluminogenic acridinium salts: A comparison study. Detection of intermediate entities appearing upon light generation. Journal of Luminescence 2017, 187 , 102-112. https://doi.org/10.1016/j.jlumin.2017.02.068
    17. Anantharajappa Kumudha, Ravi Sarada. Characterization of vitamin B12 in Dunaliella salina. Journal of Food Science and Technology 2016, 53 (1) , 888-894. https://doi.org/10.1007/s13197-015-2005-y
    18. Fei Teng, Yuri Tanioka, Natsumi Hamaguchi, Tomohiro Bito, Shigeo Takenaka, Yukinori Yabuta, Fumio Watanabe. Determination and characterization of vitamin B12 compounds in edible sea snails, ivory shell Babylonia japonica and turban shell Turdo Batillus cornutus. Fisheries Science 2015, 81 (6) , 1105-1111. https://doi.org/10.1007/s12562-015-0920-5
    19. Anantharajappa Kumudha, Sagaya Selvakumar, Pullancheri Dilshad, Gopal Vaidyanathan, Munna Singh Thakur, Ravi Sarada. Methylcobalamin – A form of vitamin B12 identified and characterised in Chlorella vulgaris. Food Chemistry 2015, 170 , 316-320. https://doi.org/10.1016/j.foodchem.2014.08.035
    20. D. Gille, A. Schmid. Vitamin B12 in meat and dairy products. Nutrition Reviews 2015, 73 (2) , 106-115. https://doi.org/10.1093/nutrit/nuu011
    21. Bhawani Chamlagain, Minnamari Edelmann, Susanna Kariluoto, Velimatti Ollilainen, Vieno Piironen. Ultra-high performance liquid chromatographic and mass spectrometric analysis of active vitamin B12 in cells of Propionibacterium and fermented cereal matrices. Food Chemistry 2015, 166 , 630-638. https://doi.org/10.1016/j.foodchem.2014.06.068
    22. M. Ovalle, E. Arroyo, M. Stoytcheva, R. Zlatev, L. Enriquez, A. Olivas. An amperometric microbial biosensor for the determination of vitamin B 12. Analytical Methods 2015, 7 (19) , 8185-8189. https://doi.org/10.1039/C5AY01599E
    23. Fan Fan Yang, Li Xin Zhu, Ren Rong Liu, Long Xu, Meng Wei. Synthesis of Vitamin B12 Artificial Antigen and Determination of Hapten Coupling Ratio. Advanced Materials Research 2014, 955-959 , 1009-1012. https://doi.org/10.4028/www.scientific.net/AMR.955-959.1009
    24. Antonella Dalle Zotte, Marco Cullere, Alberto Sartori, Alessandro Dal Bosco, Zsolt Gerencsér, Zsolt Matics, Melinda Kovàcs, Zsolt Szendrő. Effect of dietary supplementation of spirulina (Arthrospira platensis) and thyme (Thymus vulgaris) on carcass composition, meat physical traits, and vitamin B12 content on growing rabbits. World Rabbit Science 2014, 22 (1) , 11. https://doi.org/10.4995/wrs.2014.1449
    25. E. Wolfgang Becker. Microalgae for Human and Animal Nutrition. 2013, 461-503. https://doi.org/10.1002/9781118567166.ch25
    26. Ammar Ali Hassan, Torkjel M. Sandanger, Magritt Brustad. Selected Vitamins and Essential Elements in Meat from Semi-Domesticated Reindeer (Rangifer tarandus tarandus L.) in Mid- and Northern Norway: Geographical Variations and Effect of Animal Population Density. Nutrients 2012, 4 (7) , 724-739. https://doi.org/10.3390/nu4070724
    27. Fumio Watanabe, Yukinori Yabuta. Microbiological Detection of Vitamin B 12 and Other Vitamins. 2011, 165-171. https://doi.org/10.1002/9783527634156.ch13
    28. Sagaya Selva Kumar, Raghuraj Singh Chouhan, Munna Singh Thakur. Trends in analysis of vitamin B12. Analytical Biochemistry 2010, 398 (2) , 139-149. https://doi.org/10.1016/j.ab.2009.06.041
    29. Toshiaki WATANABE, Kyoko SUEMURA, Ayumi TANIGUCHI, Shuhei EBARA, Sachiko KIMURA, Toru FUKUI. Dietary Intake of Seven B Vitamins Based on a Total Diet Study in Japan. Journal of Nutritional Science and Vitaminology 2010, 56 (5) , 279-286. https://doi.org/10.3177/jnsv.56.279
    30. Reyes Barberá, Rosaura Farré, María Jesús Lagarda, Amparo Alegría. Vitamins. 2008, 291-326. https://doi.org/10.1201/9781420045338.ch15
    31. Yanling Gao, Fei Guo, Sumangala Gokavi, Andrew Chow, Qinghai Sheng, Mingruo Guo. Quantification of water-soluble vitamins in milk-based infant formulae using biosensor-based assays. Food Chemistry 2008, 110 (3) , 769-776. https://doi.org/10.1016/j.foodchem.2008.03.007
    32. Yuri Tanioka, Yukinori Yabuta, Emi Miyamoto, Hiroshi Inui, Fumio Watanabe. Analysis of Vitamin B 12 in Food by Silica Gel 60 TLC and Bioautography with Vitamin B 12 -Dependent Escherichia coli 215. Journal of Liquid Chromatography & Related Technologies 2008, 31 (13) , 1977-1985. https://doi.org/10.1080/10826070802197453
    33. . Vitamin B12. 2007, 507-534. https://doi.org/10.1201/9781420009750.ch11
    34. Fumio Watanabe. Vitamin B 12 Sources and Bioavailability. Experimental Biology and Medicine 2007, 232 (10) , 1266-1274. https://doi.org/10.3181/0703-MR-67
    35. Isabelle Ortigues-Marty, Emilie Thomas, D.P. Prévéraud, Christiane L. Girard, D. Bauchart, D. Durand, A. Peyron. Influence of maturation and cooking treatments on the nutritional value of bovine meats: Water losses and vitamin B12. Meat Science 2006, 73 (3) , 451-458. https://doi.org/10.1016/j.meatsci.2006.01.003
    36. Isabelle Ortigues-Marty, Didier Micol, Sophie Prache, Dominique Dozias, Christiane L. Girard. Nutritional value of meat: the influence of nutrition and physical activity on vitamin B12 concentrations in ruminant tissues. Reproduction Nutrition Development 2005, 45 (4) , 453-467. https://doi.org/10.1051/rnd:2005038
    37. Maciej Adamczyk, Donald D Johnson, Phillip G Mattingly, Jeffrey A Moore, You Pan. Intrinsic factor-mediated modulation of cyanocobalamin–N-sulfonyl-acridinium-9-carboxamide chemiluminescence. Bioorganic & Medicinal Chemistry Letters 2004, 14 (15) , 3917-3921. https://doi.org/10.1016/j.bmcl.2004.05.062
    38. Fumio Watanabe, Toshihide Michihata, Shigeo Takenaka, Hiromi Kittaka‐Katsura, Toshiki Enomoto, Emi Miyamoto, Satoko Adachi. Purification and Characterization of Corrinoid Compounds from a Japanese Fish Sauce. Journal of Liquid Chromatography & Related Technologies 2004, 27 (13) , 2113-2119. https://doi.org/10.1081/JLC-120039422
    39. Shigeo Takenaka, Toshiki Enomoto, Shingo Tsuyama, Fumio Watanabe. TLC Analysis of Corrinoid Compounds in Fish Sauce. Journal of Liquid Chromatography & Related Technologies 2003, 26 (16) , 2703-2707. https://doi.org/10.1081/JLC-120024540
    40. Shigeo TAKENAKA, Keiko TAKUBO, Fumio WATANABE, Toshihiko TANNO, Shingo TSUYAMA, Yoshihisa NANANO, Yoshiyuki TAMURA. Occurrence of Coenzyme Forms of Vitamin B12 in a Cultured Purple Laver (Porphyla yezoensis). Bioscience, Biotechnology, and Biochemistry 2003, 67 (11) , 2480-2482. https://doi.org/10.1271/bbb.67.2480
    41. Fumio Watanabe, Emi Miyamoto. TLC SEPARATION AND ANALYSIS OF VITAMIN B 12 AND RELATED COMPOUNDS IN FOOD. Journal of Liquid Chromatography & Related Technologies 2002, 25 (10-11) , 1561-1577. https://doi.org/10.1081/JLC-120005704
    42. Emi MIYAMOTO, Fumio WATANABE, Hiroyuki TAKENAKA, Yoshihisa NAKANO. Uptake and physiological function of vitamin B 12 in a photosynthetic unicellular coccolithophorid alga, Pleurochrysis carterae. Bioscience, Biotechnology, and Biochemistry 2002, 66 (1) , 195-198. https://doi.org/10.1271/bbb.66.195
    43. Shigeo Takenaka, Sumi Sugiyama, Shuhei Ebara, Emi Miyamoto, Katsuo Abe, Yoshiyuki Tamura, Fumio Watanabe, Shingo Tsuyama, Yoshihisa Nakano. Feeding dried purple laver (nori) to vitamin B 12 -deficient rats significantly improves vitamin B 12 status. British Journal of Nutrition 2001, 85 (6) , 699-703. https://doi.org/10.1079/BJN2001352
    44. Fumio WATANABE, Shigeo TAKENAKA, Hiromi KATSURA, Emi MIYAMOTO, Katsuo ABE, Yoshiyuki TAMURA, Toshiyuki NAKATSUKA, Yoshihisa NAKANO. Characterization of a Vitamin B12 Compound in the Edible Purple Laver, Porphyra yezoensis.. Bioscience, Biotechnology, and Biochemistry 2000, 64 (12) , 2712-2715. https://doi.org/10.1271/bbb.64.2712
    Download PDF

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    Pair your accounts.

    Export articles to Mendeley

    Get article recommendations from ACS based on references in your Mendeley library.

    You’ve supercharged your research process with ACS and Mendeley!

    STEP 1:
    Click to create an ACS ID

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

    MENDELEY PAIRING EXPIRED
    Your Mendeley pairing has expired. Please reconnect