Abstract
It has been previously demonstrated that the conditioning therapy given to bone marrow transplant (BMT) recipients creates a high oxidant stress, resulting in a measured reduction in antioxidants, such as glutathione peroxidase (GSH-Px), vitamin E, and cell peroxide fragilities.
As part of a current intervention trial of antioxidant therapy in BMT recipients, plasma thiobarbituric acid reactive substances (TBARS) were measured to assess peroxidation and free radical activity. Measurements were performed before and after conditioning therapy, and then at weekly intervals for a period of 6 wk after transplantation in 20 patients (10 controls and 10 antioxidant therapy [AOT] recipients). The TBARS results were compared with concurrent measurements of more specific elements of the antioxidant pathways, such as red blood cell glutathione peroxidase (RBC-GSH-Px), plasma vitamin C, and serum vitamin E.
In all cases, TBARS concentration was significantly increased after conditioning compared with baseline levels (p<0.001), an increase that correlated inversely with RBC-GSH-Px (r=−0.81;p<0.01). The TBARS concentration fell gradually after conditioning in all patients. The fall in the AOT group was more rapid than in the control group, and it paralleled the gradual return toward normal levels of the other antioxidants. The change in TBARS concentration occurred faster than changes in other indices, suggesting that TBARS might be a better index of overall free radical activity.
Although the patient numbers are small, there is some evidence to suggest that MDA may act as a prognostic marker. In 70% of the patients with a poor postoperative course and who eventually died, the peak TBARS concentration was significantly higher than that in the successful transplants. Also, the fall in TBARS concentration was much slower (if at all). This point requires further investigation and a more detailed analysis on a larger number of patients.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
T. F. Slater,Biochem. J. 222, 1 (1984).
B. Halliwell, and J. M. C. Gutteridge,Mol. Aspects Med. 8, 89 (1985).
I. Nishigaki, M. Hagihara, H. Tsunekawa, M. Maseki, and K. Yagi,Biochem. Med. 25, 373 (1981).
M. T. Santos, J. Valles, J. Anzar, and J. Vilches,J. Clin. Path. 33, 973 (1980).
J. Kurantsin-Mills, N. Samji, M. A. Moscarello,Neurochem. Res.,7, 1523. (1982).
H. Kappus, inFree Radicals and Food Additives, O. Aruoma, and B. Halliwell, eds., Taylor and Francis in (1991).
K. H. Cheeseman, and T. F. Slater,Br. Med. Bull. 49, 481 (1993).
B. M. Babior, R. S. Kipnes, and J. T. Curnutte,J. Clin. Invest.,52, 741 (1973).
R. B. Johnson, and J. E. Lehmeyer,J. Clin. Invest.,57, 836 (1976).
T. L. Dormandy,Lancet,I, 647 (1978).
H. L. Gurtoo, J. H. Hipkens, and S. D. Sharma,Cancer Res. 41, 3584 (1981).
B. Halliwell,J. Roy. Soc. Med. 82, 747 (1989).
S. C. Purohit, R. H. Bisby, and R. B. Cundell,Int. J. Radiat. Biol. 38, 147 (1980).
K. H. Cheeseman,DNA and Free Radicals, B. Halliwell and O. Aruoma, eds., Ellis Horward, UK (1993).
H. Esterbauer, R. J. Salauer, and H. Zollner,Free Radic. Biol. Med. 11, 87 (1991).
E. P. Corongui, G. Poli, M. U. Dianzani, K. H. Cheesman, and T. F. Slater,Chem. Biol. Interactions 59, 147 (1976).
C. V. Smith, and R. E. Anderson,Free Radic. Biol. Med. 3, 341 (1987).
K. Satoh,Clin. Chim. Acta 90, 37 (1978).
K. Yagi,Biochem. Med. 15, 212 (1976).
S. H. Y. Wong, J. A. Knight, S. M. Hopfer, O. Zaharia, C. N. Leach, and F. W. Sundermann,Clin. Chem. 33, 214 (1987).
M. Conti, P. C. Morand, P. Levillain, and A. Lemmonier,Clin. Chem. 37, 1273 (1991).
M. A. Carbonneau, E. Peuchant, D. Sess, P. Canioni, and M. Clerc,Clin. Chem. 37, 1423 (1991).
H. A. Cynamon, J. N. Isenberg, and C. O. Neuyen,Clin. Chim. Acta 151, 169 (1985).
A. Szczeklik, R. J. Gryelewski, B. Domagala, A. Zmuda, J. Harwick, E., Wozny, M. Grzywacz, J. Madej, and T. Gryglewska,Prostaglandins 22, 795 (1981).
M. Mihara, M. Uehiyama, and K. Fukuzawa,Biochem. Med. 23, 302 (1980).
H. Varley inPractical Clinical Chemistry (3rd ed.), William Heinemann Medical, London, p. 524 (1962).
L. Catignani, and J. G. Bieri,Clin. Chem. 29, 708 (1983).
P. A. Pleiban, A. Munyani, and J. Beechum,Clin. Chem. 28, 311 (1981).
S. S. Donaldson, and R. A. Lenon,Cancer,43, 2036 (1979).
M. R. Clemens, C. Ladner, G. Ehninger, H. Einsele, W. Renn, E. Buhler, H. Waller, and K. F. Gey,Am. J. Clin. Nutr. 51, 216 (1990).
A. G. Hunnisett, A. Kars, J. Howard, and S. Davies,Amino Acids 4, 177 (1993).
E. P. Bird, and H. H. Draper,Methods Enzymol. 105, 299 (1984).
M. A. Q. Arshad, S. Bradra, R. M. Cohen, and M. T. R. Subbiah,Clin. Chem.,37, 1756 (1991).
V. K. S. Shukla, J. E. Jensen, and J. Clausen,Acta Neurol. Scand.,56, 542 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hunnisett, A., Davies, S., McLaren-Howard, J. et al. Lipoperoxides as an index of free radical activity in bone marrow transplant recipients. Biol Trace Elem Res 47, 125–132 (1995). https://doi.org/10.1007/BF02790109
Issue Date:
DOI: https://doi.org/10.1007/BF02790109