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Effects of combination of melatonin and laser irradiation on ovarian cancer cells and endothelial lineage viability

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Abstract

The main goal of anti-cancer therapeutic approaches is to induce apoptosis in tumor masses but not in the normal tissues. Nevertheless, the combination of photodynamic irradiation with complementary oncostatic agents contributes to better therapeutic performance. Here, we applied two different cell lines; SKOV3 ovarian carcinoma cells and HUVECs umbilical cord cells as in vitro models to pinpoint whether pharmacological concentration of melatonin in combination with photodynamic therapy induces cell cytotoxicity. The cells were separately treated with various concentrations of melatonin (0 to 10 mM) and photodynamic irradiation alone or in combination. Cells were preliminary exposed to increasing concentrations of melatonin for 24 h and subsequently underwent laser irradiation for 60 s with an output power of 80 mW in continuous mode at 675 nm wavelength and a total light dose of 13.22 J/cm2. Cell viability, apoptosis/necrosis rates, and reactive oxygen species levels as well as heat shock protein 70 expression were monitored after single and combined treatments. A statistical analysis was performed by applying one-way analysis of variance (ANOVA) and post hoc Tukey’s test. Combination treatment of both cell lines caused a marked increase in apoptosis/necrosis rate, reactive oxygen species generation, and heat shock protein 70 expression compared to incubation of the cells with each agent alone (p < 0.05). SKOV3 cancer cells expressed higher level of heat shock protein 70 under experimental procedure as compared to HUVECs (p < 0.05). Our results introduce melatonin as a potent stimulus for enhancing the efficacy of laser on induction of apoptosis in tumor cells.

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    Authors and Affiliations

    1. Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran

      Maryam Akbarzadeh, Mohammad Nouri, Maryam Vahidi Banekohal, Omid Cheraghi, Sina Soltani, Navid Feizy, Soheila Montazersaheb, Reza Rahbarghazi & Nasser Samadi

    2. Department of Clinical Biochemistry and Laboratory Medicine, Tabriz University of Medical Sciences, Imam Reza St., Golgasht St., 5166614756, Tabriz, Iran

      Maryam Akbarzadeh, Navid Feizy & Nasser Samadi

    3. Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

      Omid Cheraghi

    4. Research Institute for Applied Physics and Astronomy, University of Tabriz, Tabriz, Iran

      Habib Tajalli

    5. Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

      Aliakbar Movassaghpour

    6. Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

      Hadi Cheraghi

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    1. Maryam Akbarzadeh
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    Correspondence to Reza Rahbarghazi or Nasser Samadi.

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    Reza Rahbarghazi and Nasser Samadi contributed equally to this work.

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    Akbarzadeh, M., Nouri, M., Banekohal, M.V. et al. Effects of combination of melatonin and laser irradiation on ovarian cancer cells and endothelial lineage viability. Lasers Med Sci 31, 1565–1572 (2016). https://doi.org/10.1007/s10103-016-2016-6

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    • DOI: https://doi.org/10.1007/s10103-016-2016-6

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