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Parthenolide attenuates 7,12-dimethylbenz[a]anthracene induced hamster buccal pouch carcinogenesis

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Abstract

Over the decades, the survival rates for oral cancer have not improved despite development in novel diagnostic and therapeutic strategies. Therefore, the present study is aimed at investigating the chemopreventive potential of parthenolide in DMBA-induced hamster buccal pouch carcinogenesis. The hamsters were divided into 4 groups (n = 6/group). Group I was treated as control. Groups II and III were painted with a solution of 0.5% DMBA three times per week for 14 weeks on the left buccal pouches. In addition, group III were orally administrated with parthenolide 2 mg/kg b.w on days alternate to the DMBA application. Group IV received only parthenolide. At the end of 14th week all hamsters were sacrificed. Buccal tissues from all hamsters were evaluated for histopathology. Biochemical studies were carried out using plasma, liver, and buccal mucosa of control and experimental hamsters. Gene and protein expression studies of apoptotic markers p53, Bcl-2, and Bax were performed. The results showed 100% tumor formation and marked alterations in histopathology, status of detoxification enzymes, lipid peroxidation, and antioxidant profile in group II hamsters. Oral administration of parthenolide completely prevented tumor formation and significantly reduced the severity of histopathological changes in group III hamsters. The status of detoxification enzymes, lipid peroxidation, and antioxidants were significantly restored in parthenolide treated group compared to group II hamsters. The apoptotic gene p53 and antiapoptotic gene Bcl-2 were significantly down regulated; whereas, pro-apoptotic gene Bax was up regulated in group III hamsters compared to group II. The results of the present study suggest that parthenolide have potent chemopreventive, antioxidant, and apoptotic effect in DMBA-induced oral carcinogenesis.

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Acknowledgements

Financial support from University Grants Commission (UGC), New Delhi to Dr. N. Baskaran in the form of UGC-Post Doctoral Fellow (Ref: No. F./PDFSS-2013-14-SC-TAM-4741 dt. On 03/07/2014) is gratefully acknowledged. Further, Author gratefully acknowledged to Dr.G.S.Selvam, Professor and Head, Department of Biochemistry, Madurai Kamaraj University, Madurai for his valuable guidance.

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  1. Cancer Biology Unit, Department of Biochemistry, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, 625 021, India

    Nagarethinam Baskaran, Govindan Sadasivam Selvam, Subramani Yuvaraj & Albert Abhishek

  2. Department of Chemistry and Biosciences, SASTRA University, Srinivasa Ramanujan Centre (SRC), Kumbakonam, Tamil Nadu, 612001, India

    Nagarethinam Baskaran

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Baskaran, N., Selvam, G.S., Yuvaraj, S. et al. Parthenolide attenuates 7,12-dimethylbenz[a]anthracene induced hamster buccal pouch carcinogenesis. Mol Cell Biochem 440, 11–22 (2018). https://doi.org/10.1007/s11010-017-3151-5

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