Andrologia

Volume 48, Issue 2 p. 143-151

Original Article

Protective effect of thymoquinone against testicular torsion induced oxidative injury

M. Ayan,

M. Ayan

Department of Emergency Medicine, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

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U. Tas,

U. Tas

Department of Anatomy, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

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E. Sogut,

E. Sogut

Department of Biochemistry, Faculty of Medicine, Katip Celebi University, İzmir, Turkey

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S. Caylı,

S. Caylı

Department of Histology and Embryology, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey

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H. Kaya,

H. Kaya

Department of Emergency Medicine, Faculty of Medicine, Harran University, Urfa, Turkey

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M. Esen,

M. Esen

Department of Emergency Medicine, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

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F. Erdemir,

F. Erdemir

Department of Urology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

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M. Uysal,

Corresponding Author

M. Uysal

Department of Anatomy, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

Correspondence

Assoc. Prof. Murat Uysal, MD, Department of Anatomy, Gaziosmanpasa University, Tokat, Turkey.

Tel.: +90 5319314700;

Fax.: +903562133179

E-mail: [email protected]

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M. Ayan,

M. Ayan

Department of Emergency Medicine, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

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U. Tas,

U. Tas

Department of Anatomy, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

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E. Sogut,

E. Sogut

Department of Biochemistry, Faculty of Medicine, Katip Celebi University, İzmir, Turkey

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S. Caylı,

S. Caylı

Department of Histology and Embryology, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey

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H. Kaya,

H. Kaya

Department of Emergency Medicine, Faculty of Medicine, Harran University, Urfa, Turkey

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M. Esen,

M. Esen

Department of Emergency Medicine, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

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F. Erdemir,

F. Erdemir

Department of Urology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

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M. Uysal,

Corresponding Author

M. Uysal

Department of Anatomy, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

Correspondence

Assoc. Prof. Murat Uysal, MD, Department of Anatomy, Gaziosmanpasa University, Tokat, Turkey.

Tel.: +90 5319314700;

Fax.: +903562133179

E-mail: [email protected]

Search for more papers by this author

First published: 23 April 2015

https://doi.org/10.1111/and.12424

Citations: 32

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Summary

We aimed to determine the protective effects of thymoquinone (TQ), against ischaemia–reperfusion (I/R) injury in the testis tissue of rats. Twenty-seven male Wistar albino rats were randomly divided into three equal groups as follows: Group I, sham group; Group II, torsion group; and Group III, torsion + thymoquinone group. The ischaemia period was 2 h, and orchiectomy was performed after 30 min of detorsion. Testis tissue sections were analysed with the terminal transferase mediated dUTP-nick end labelling (TUNEL) assay to determine in situ apoptotic DNA fragmentation. Additionally, Caspase 3 and Bax proteins were analysed immunohistochemically. The superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) activity levels in the testis tissue were also measured. The superoxide dismutase activity and malondialdehyde levels in the torsion group were significantly higher than those of the sham group (P < 0.05). Thymoquinone administration significantly reduced these levels. Torsion significantly increased active-Caspase 3 and Bax expression, which was decreased by thymoquinone. The apoptotic index of the torsion group was significantly higher than that of the control group. However, thymoquinone significantly reduced the apoptotic index (P < 0.05). Our results indicate that thymoquinone plays a protective role in oxidative stress induced ischaemia–reperfusion in the testis tissue of rats.

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Volume48, Issue2

March 2016

Pages 143-151

Protective effect of thymoquinone against testicular torsion induced oxidative injury

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Protective effect of thymoquinone against testicular torsion induced oxidative injury

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