Effect of fucoidan from Turbinaria conoides on human lung adenocarcinoma epithelial (A549) cells

Carbohydr Polym. 2016 Nov 5:152:207-213. doi: 10.1016/j.carbpol.2016.06.112. Epub 2016 Jul 7.

Abstract

Fucoidan was purified from seaweed, Turbinaria conoides. Isolated fragments were characterized with NMR ((13)C, (1)H), Gas Chromatography-Mass Spectronomy (GC-MS) and HPLC analysis. The autohydrolysate of fucoidans consisted of sulfated fuco-oligosaccharides having the backbone of α-(1, 3)-linked fuco-pyranose derivatives and minor components of galactose, glucose, mannose and xylose sugars. Fucoidan induced a dose-dependent reduction in cell survival of lung cancer A549 cells by MTT assay (GI50, 75μg/mL). However, it was not cytotoxic to a non-tumorigenic human keratinocyte cell line of skin tissue (HaCaT) (GI50>1.0mg/mL). The apoptotic cells in fucoidan-treated A549 cells were visualized by laser confocal microscopy and cell cycle analysis showed induction of G0/G1 phase arrest of the cell progression cycle. Further, CFSE labeling and flow cytometry highlighted that fucoidan significantly (P<0.05) inhibited the proliferation rate of A549 cells by up to 2-fold compared with the control cells. It is concluded that fucoidan has the potential to act as an anti-proliferative agent on lung carcinoma (A549) cells.

Keywords: Apoptosis; CFSE labeling; Fucoidan (PubChem CID: 92023653); G0/G1 arrest; Structural analysis.

MeSH terms

  • A549 Cells
  • Adenocarcinoma / pathology*
  • Adenocarcinoma of Lung
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Drug Screening Assays, Antitumor
  • Epithelial Cells / drug effects*
  • Epithelial Cells / pathology
  • Humans
  • Lung Neoplasms / pathology*
  • Phaeophyceae / chemistry
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Respiratory Mucosa / drug effects*
  • Respiratory Mucosa / pathology
  • Seaweed / chemistry

Substances

  • Antineoplastic Agents
  • Polysaccharides
  • fucoidan