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Antioxidant activity of fucose-containing sulfated polysaccharides obtained from Fucus evanescens by different extraction methods

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Abstract

The fucose-containing sulfated polysaccharides (FCSPs) from Fucus evanescens were obtained via different procedures: by hot acidic extraction and with aqueous calcium chloride solution, followed by separation FCSPs using anion-exchanging chromatography. Chemical characteristics of these polysaccharide fractions were determined. It was shown that extraction with aqueous calcium chloride solution provides a purified preparation of the FCSPs. The crude polysaccharide and its chromatography purified fractions obtained by this method have significantly lower contents of laminaran, uronic acids, and polyphenols in comparison with FCSPs obtained by hot acid extraction. All polysaccharides exhibited total antioxidant capacity and 1,1-diphenyl-2-pycrilhydrazyl (DPPH) radical-scavenging activity and low 2,2′-azino-bis 3-ethyl benzothiazoline-6-sulfuric acid (ABTS) radical-scavenging activity. A strong positive correlation between polyphenol content in FCSPs and their antioxidant activity was found. This fact may indicate that phenols play a key role in the previously described antioxidant capacity of FCSPs.

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Acknowledgments

This study was supported by the Russian Foundation for Basic Research (projects 12-04-0069 and 14-04-00973) and the RAS Program on Molecular and Cell Biology.

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Authors and Affiliations

  1. Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690022, Russia

    Tatiana I. Imbs & Tatiana N. Zvyagintseva

  2. A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia

    Anna V. Skriptsova

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  1. Tatiana I. Imbs
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  2. Anna V. Skriptsova
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  3. Tatiana N. Zvyagintseva
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Correspondence to Tatiana I. Imbs.

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Imbs, T.I., Skriptsova, A.V. & Zvyagintseva, T.N. Antioxidant activity of fucose-containing sulfated polysaccharides obtained from Fucus evanescens by different extraction methods. J Appl Phycol 27, 545–553 (2015). https://doi.org/10.1007/s10811-014-0293-7

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  • DOI: https://doi.org/10.1007/s10811-014-0293-7

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