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Astragalus membranaceus: A Review of its Protection Against Inflammation and Gastrointestinal Cancers

    https://doi.org/10.1142/S0192415X16500014Cited by:233 (Source: Crossref)
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    Abstract

    Astragalus membranaceus is a major medicinal herb commonly used in many herbal formulations in the practice of traditional Chinese medicine (TCM) to treat a wide variety of diseases and body disorders. Among its diversified clinical applications, the potential use of this herb and its chemical constituents in treatments of inflammatory diseases and cancers has been actively investigated in recent years. Astragalus-based treatments have demonstrated significant amelioration of the toxicity induced by other concurrently administered orthodox drugs (e.g., immunosuppressants and cancer chemotherapeutics). The major components of Astragalus membranaceus are polysaccharides, flavonoids, and saponins. Contemporary use of Astragalus membranaceus mainly focuses on its immunomodulating, anti-oxidant, and anti-inflammatory, as well as anticancer effects. In this paper, we summarize the properties of Astragalus membranaceus and its major constituents in the biological system based on experimental and clinical studies. The antitumorigenic mechanisms of a novel Astragalus saponins extract called AST in treating various gastrointestinal cancers are highlighted. We discuss in detail how the Astragalus herb and AST influence the immune system, modulate various cancer signaling pathways, and interact with specific transcription molecules during protection against gastrointestinal inflammation and cancers. This information could help clinicians and scientists develop novel target-specific and effective therapeutic agents that are deprived of major systemic side effects, so as to establish a better treatment regimen in the battle against inflammatory diseases and cancers of the gut.

    References

    • Anonymous. Astragalus membranaceus. Monograph. Altern. Med. Rev. 8(1): 72–77, 2003. Google Scholar
    • Auyeung, K.K., C.H. Cho and J.K. Ko [2009a] A novel anticancer effect of Astragalus saponins: Transcriptional activation of NSAID-activated gene Int. J. Cancer 125 (5), 1082–1091. Medline, ISI, Google Scholar
    • Auyeung, K.K. and J.K. Ko [2010] Novel herbal flavonoids promote apoptosis but differentially induce cell cycle arrest in human colon cancer cell. Invest. New Drugs 28 (1), 1–13. Medline, ISI, Google Scholar
    • Auyeung, K.K., P.C. Law and J.K. Ko [2009] Astragalus saponins induce apoptosis via an ERK-independent NF-κB signaling pathway in the human hepatocellular HepG2 cell line Int. J. Mol. Med. 23 (2), 189–196. Medline, ISI, Google Scholar
    • Auyeung, K.K., P.C. Law and J.K. Ko [2014] Combined therapeutic effects of vinblastine and Astragalus saponins (AST) in human colon cancer cells and tumor xenograft via inhibition of tumor growth and proangiogenic factors Nutr. Cancer 66 (4), 662–674. Medline, ISI, Google Scholar
    • Auyeung, K.K., P.C. Law and J.K. Ko [2012a] Novel anti-angiogenic effects of formononetin in human colon cancer cells and tumor xenograft Oncol. Rep. 28 (6), 2188–2194. Medline, ISI, Google Scholar
    • Auyeung, K.K., P.K. Woo, P.C. Law and J.K. Ko [2012b] Astragalus saponins modulate cell invasiveness and angiogenesis in human gastric adenocarcinoma cells J. Ethnopharmacol. 141 (2), 635–641. Medline, ISI, Google Scholar
    • Auyeung, K.K.W., P.C. Law, L.Y. Chan and J.K.S. Ko [2013] Astragalus saponins downregulate hypoxia-induced VEGF induction in colon cancer cells BMC Comp. Altern. Med. 12, 160. ISI, Google Scholar
    • Baek, S.J., J.S. Kim, F.R. Jackson, T.E. Eling, M.F. McEntee and S.H. Lee [2004] Epicatechin gallate-induced expression of NAG-1 is associated with growth inhibition and apoptosis in colon cancer cells. Carcinogenesis 25 (12), 2425–2432. Medline, ISI, Google Scholar
    • Bensky, D. and A. Gamble [1993] Chinese Herbal Medicine: Materia Medica, Revised Edition. Seattle: Eastland Press. Google Scholar
    • Bian, Y.Y., J. Guan, Z.M. Bi, Y. Song and P. Li [2006] Studies on chemical constituents of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao Chin. Pharm. J. 41, 1217–1221. Google Scholar
    • Block, K.I. and M.N. Mead [2003] Immune system effects of echinacea, ginseng, and astragalus: A review. Integr. Cancer Ther. 2 (3), 247–267. Medline, Google Scholar
    • Chang, H. and P. But [1987] Pharmacology and Applications of Chinese Materia Medica, 2 Singapore: World Scientific, pp. 1041–1046. Link, Google Scholar
    • Chen, J.K. and T.T. Chen [2004] Chinese Medical Herbology and Pharmacology. Los Angeles: Art of Medicine Press. Google Scholar
    • Chen, L.J., M.L. Shen, M.Y. Wang, S.K. Zhai and M.Z. Liu [1981] Effect of Astragalus polysaccharides on phagocytic function in mice (in Chinese). Zhongguo Yao Li Xue Bao 2 (3), 200–204. Medline, Google Scholar
    • Chen, L.X., J.Z. Liao and W.Q. Guo [1995] Effects of Astragalus membranaceus on left ventricular function and oxygen free radical in acute myocardial infarction patients and mechanism of its cardiotonic action (in Chinese) Zhongguo Zhong Xi Yi Jie He Za Zhi 15 (3), 141–143. Medline, Google Scholar
    • Cho, W.C. and K.N. Leung [2007a] In vitro and in vivo anti-tumor effects of Astragalus membranaceus Cancer Lett. 252 (1), 43–54. Medline, ISI, Google Scholar
    • Cho, W.C. and K.N. Leung [2007b] In vitro and in vivo immunomodulating and immunorestorative effects of Astragalus membranaceus J. Ethnopharmacol. 113 (1), 132–141. Medline, ISI, Google Scholar
    • Chou, J., S.J. Liu, J.W. Lin and S.L. Wang [2007] Clinical application of Radix Astragali injection (i.v.) reduced toxicity from cancer chemotherapy Zhe Jiang Zhong Yi Za Zhi 42, 179–180. Google Scholar
    • Chu, C., H.X. Cai, M.T. Ren, E.H. Liu, B. Li, L.W. Qi and P. Li [2010] Characterization of novel astragaloside malonates from Radix Astragali by HPLC with ESI quadrupole TOF MS J. Sep. Sci. 33 (4–5), 570–581. Medline, ISI, Google Scholar
    • Chu, D.T., W.L. Wong and G.M. Mavligit [1988] Immunotherapy with Chinese medicinal herbs. II. Reversal of cyclophosphamide-induced immune suppression by administration of fractionated Astragalus membranaceus in vivo. J. Clin. Lab. Immunol. 25 (3), 125–129. Medline, ISI, Google Scholar
    • Cui, R., J. He, B. Wang, F. Zhang, G. Chen, S. Yin and H. Shen [2003] Suppressive effect of Astragalus membranaceus Bunge on chemical hepatocarcinogenesis in rats Cancer Chemother. Pharmacol. 51 (1), 75–80. Medline, ISI, Google Scholar
    • Du, Q., Z. Chen, L.F. Zhou, Q. Zhang, M. Huang and K.S. Yin [2008] Inhibitory effects of astragaloside IV on ovalbumin-induced chronic experimental asthma. Can. J. Physiol. Pharmacol. 86 (7), 449–457. Medline, ISI, Google Scholar
    • Duan, P. and Z.M. Wang [2002] Clinical study on effect of Astragalus in efficacy enhancing and toxicity reducing of chemotherapy in patients of malignant tumor (in Chinese). Zhongguo Zhong Xi Yi Jie He Za Zhi. 22 (7), 515–517. Medline, Google Scholar
    • Ellington, A.A., M. Berhow and K.W. Singletary [2005] Induction of macroautophagy in human colon cancer cells by soybean B–group triterpenoid saponins. Carcinogenesis 26 (1), 159–167. Medline, ISI, Google Scholar
    • Fang, J.N. and H. Wagner [1998] Chemical structure of a glucan from Astragalus mongholicus Acta Chimica Sinica 46, 1101–1104. Google Scholar
    • He, Z.Q. and J. Findlay [1991] Constituents of Astragalus membranaceous J. Nat. Prod. 54, 810–815. ISI, Google Scholar
    • He, Z.Q. and B.Q. Wang [1990] Isolation and identification of chemical constituents of Astragalus root. Acta Pharm. Sinica 25 (9), 694–698. Google Scholar
    • Hei, Z.Q., J.J. Zhang, S.Q. Lin, K.X. Liu and B.X. Chen [2004] Effects of Astragalus membranaceus injection on nitric oxide and endothelin concentration of intestinal mucosa after hemorrhage shock–reperfusion in rats (in Chinese) Zhongguo Zhong Yao Za Zhi 29 (5), 444–447. Medline, Google Scholar
    • Hikino, H., S. Funayama and K. Endo [1976] Hypotensive principle of Astragalus and Hedysarum roots. Planta Med. 30 (4), 297–302. Medline, ISI, Google Scholar
    • Hong, Y. [1986] Oriental Materica: A Concise Guide.. Long Beach: Oriental Healing Arts Institute. Google Scholar
    • Huang, K.C. [1999] The Pharmacology of Chinese Herbs.. Boca Raton: CRC Press. Google Scholar
    • Ji, D.B., J. Ye, Y.M. Jiang and B. Qian [2009] Anti-tumor effect of Liqi: A traditional Chinese medicine prescription, in tumor bearing mice. BMC Comp. Altern. Med. 9, 20. Medline, ISI, Google Scholar
    • Jiao, Y., J. Wen and X. Yu [1999] Influence of flavonoid of Astragalus membranaceus’s stem and leaves on the function of cell mediated immunity in mice (in Chinese) Zhongguo Zhong Xi Yi Jie He Za Zhi 19 (6), 356–358. Medline, Google Scholar
    • Kang, J.H., I.H. Han, M.K. Sung, H. Yoo, Y.G. Kim, J.S. Kim, T. Kawada and R. Yu [2008] Soybean saponin inhibits tumor cell metastasis by modulating expressions of MMP-2, MMP-9 and TIMP- 2. Cancer Lett. 261 (1), 84–92. Medline, ISI, Google Scholar
    • Kao, S.T., C.C. Yeh, C.C. Hsieh, M.D. Yang, M.R. Lee, H.S. Liu and J.G. Lin [2001] The Chinese medicine Bu-Zhong-Yi-Qi-Tang inhibited proliferation of hepatoma cell lines by inducing apoptosis via G0/G1 arrest. Life Sci. 69 (13), 1485–1496. Medline, ISI, Google Scholar
    • Kitagawa, I., D. Wang and M. Yoshikawa [1983] Saponin and sapogenol. XXXVII: Chemical constituents of Astragali Radix, the root of Astragalus membranaceus Bunge. (4): Astragalosides VII and VIII Chem. Pharm. Bull. (Tokyo) 31, 716–722. ISI, Google Scholar
    • Kiyohara, H., T. Uchida, M. Takakiwa, T. Matsuzaki, N. Hada, T. Takeda, T. Shibata and H. Yamada [2010] Phytochemistry 71 (2–3), 280–293. Medline, ISI, Google Scholar
    • Ko, J.K. and K.K.W. Auyeung [2013] Target–oriented mechanisms of novel herbal therapeutics in the chemotherapy of gastrointestinal cancer and inflammation. Curr. Pharm. Design 19 (1), 48–66. Medline, ISI, Google Scholar
    • Ko, J.K. and C.W. Chik [2009] The protective action of radix Astragalus membranaceus against hapten-induced colitis through modulation of cytokines Cytokine 47 (2), 85–90. Medline, ISI, Google Scholar
    • Ko, J.K., F.Y. Lam and A.P. Cheung [2005] Amelioration of experimental colitis by Astragalus membranaceus through anti-oxidation and inhibition of adhesion molecule synthesis World J. Gastroenterol. 11 (37), 5787–5794. Medline, ISI, Google Scholar
    • Konkimalla, V.B. and T. Efferth [2008] Evidence–based Chinese medicine for cancer therapy. J. Ethnopharmacol. 116 (2), 207–210. Medline, ISI, Google Scholar
    • Kurashige, S., Y. Akuzawa and F. Endo [1999] Effects of astragali radix extract on carcinogenesis, cytokine production, and cytotoxicity in mice treated with a carcinogen, N–butyl–N’–butanolnitrosoamine. Cancer Invest. 17 (1), 30–35. Medline, ISI, Google Scholar
    • Lau, B.H., H.C. Ruckle, T. Botolazzo and P.D. Lui [1994] Chinese medicinal herbs inhibit growth of murine renal cell carcinoma. Cancer Biother. 9 (2), 153–161. Medline, Google Scholar
    • Lee, K.Y. and Y.J. Jeon [2005] Macrophage activation by polysaccharide isolated from Astragalus membranaceus Int. Immunopharmacol. 5 (7–8), 1225–1233. Medline, ISI, Google Scholar
    • Li, C.X., L. Li, J. Lou, W.X. Yang, T.W. Lei, Y.H. Li, J. Liu, M.L. Cheng and L.H. Huang [1998] The protective effects of traditional Chinese medicine prescription, han–dan–gan–le, on CCl4–induced liver fibrosis in rats Am. J. Chin. Med. 26 (26–4), 325–332. Link, ISI, Google Scholar
    • Li, R., W. Chen, W. Wang, W. Tian and X. Zhang [2009] Extraction, characterization of Astragalus polysaccharides and its immune modulating activities in rats with gastric cancer. Carbohydrate Polymers 78, 738–742. ISI, Google Scholar
    • Li, S.G. and Y.Q. Zhang [2009] Characterization and renal protective effect of a polysaccharide from Astragalus membranaceus Carbohydr. Polym. 78, 343–348. ISI, Google Scholar
    • Li, X., D. He, L. Zhang, X. Cheng, B. Sheng and Y. Luo [2006] A novel antioxidant agent, astragalosides, prevents shock wave-induced renal oxidative injury in rabbits. Urol. Res. 34 (4), 277–282. Medline, Google Scholar
    • Lin, J., H.F. Dong, J.J. Oppenheim and O.M. Howard [2003] Effects of astragali radix on the growth of different cancer cell lines. World J. Gastroenterol. 9 (4), 670–673. Medline, ISI, Google Scholar
    • Lin, L.Z., X.G. He, M. Lindenmaier, G. Nolan, J. Yang, M. Cleary, S.X. Qiu and G.A. Cordell [2000] Liquid chromatography-electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A. membranaceus J. Chromatogr. A 876 (1–2), 87–95. Medline, ISI, Google Scholar
    • Lin, S.Y., J.D. Liu, H.C. Chang, S.D. Yeh, C.H. Lin and W.S. Lee [2002] Magnolol suppresses proliferation of cultured human colon and liver cancer cells by inhibiting DNA synthesis and activating apoptosis. J. Cell Biochem. 84 (3), 532–544. Medline, ISI, Google Scholar
    • Ma, X.Q., Q. Shi, J.A. Duan, T.T. Dong and K.W. Tsim [2002] Chemical analysis of Radix Astragali (Huangqi) in China: A comparison with its adulterants and seasonal variations. J. Agric. Food Chem. 50 (17), 4861–4866. Medline, ISI, Google Scholar
    • Matkowski, A., D. Wozniak, E. Lamer-Zarawska, J. Oszmianski and A. Leszczynska [2003] Flavonoids and phenol carboxylic acids in the oriental medicinal plant Astragalus membranaceus acclimated in Poland Z. Naturforsch. C 58 (58–8), 602–604. Medline, ISI, Google Scholar
    • McCulloch, M., C. See, X.J. Shu, M. Broffman, A. Kramer, W.Y. Fan, J. Gao, W. Lieb, K. Shieh and J.M. Colford  Jr. [2006] Astragalus-based Chinese herbs and platinum- based chemotherapy for advanced non-small-cell lung cancer: Meta-analysis of randomized trials. J. Clin. Oncol. 24 (3), 419–430. Medline, ISI, Google Scholar
    • McKenna, D.J., K. Hughes and K. Jones [2002] Astragalus. Int. J. Integr. Med. 4, 40–46. Google Scholar
    • Miller, L.G. [1998] Herbal medicinals: Selected clinical considerations focusing on known or potential drug–herb interactions. Arch. Intern. Med. 158 (20), 2200–2211. Medline, Google Scholar
    • Mills, S. and K. Bone [2000] Principles and Practice of Phytotherapy. Edinburgh: Churchill Livingstone. Google Scholar
    • Miyazawa, M. and H. Kameoka [1987] Volatile flavor components of Astragali Radix (Astragalus membranaceus Bunge). Agric. Biol. Chem. 51, 3153–3154. Google Scholar
    • Na, D., F.N. Liu, Z.F. Miao, Z.M. Du and H.M. Xu [2009] Astragalus extract inhibits destruction of gastric cancer cells to mesothelial cells by anti-apoptosis World J. Gastroenterol. 15 (5), 570–577. Medline, ISI, Google Scholar
    • Pei, Y., R. Li, H. Fu, J. Wang and Y. Zhou [2007] A new isoflavone glucoside from Astragalus membranaceus var mongholicus. Fitoterapia 78 (78–8), 602–604. Medline, ISI, Google Scholar
    • Purmova, J. and L. Opletal [1995] Saponins and possibilities of their use in prevention and therapy (translated Abstract). Ceska Slov. Farm. 44 (5), 246–251 Phytotherapeutic aspects of diseases of the cardiovascular system. 5. Medline, Google Scholar
    • Qi, L.W., Q.T. Yu, P. Li, S.L. Li, Y.X. Wang, L.H. Sheng and L. Yi [2006] Quality evaluation of Radix Astragali through a simultaneous determination of six major active isoflavonoids and four main saponins by high-performance liquid chromatography coupled with diode array and evaporative light scattering detectors J. Chromatogr. A 1134 (1–2), 162–169. Medline, ISI, Google Scholar
    • Qi, L.W., Q.T. Yu, L. Yi, M.T. Ren, X.D. Wen, Y.X. Wang and P. Li [2008] Simultaneous determination of 15 marker constituents in various radix Astragali preparations by solid-phase extraction and high-performance liquid chromatography. J. Sep. Sci. 31 (1), 97–106. Medline, ISI, Google Scholar
    • Qin, Q., J. Niu, Z. Wang, W. Xu, Z. Qiao and Y. Gu [2012] Astragalus membranaceus inhibits inflammation via phospho-p38 mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κb pathways in advanced glycation end product-stimulated macrophages Int. J. Mol. Sci. 13 (7), 8379–8387. Medline, ISI, Google Scholar
    • Shao, B.M., W. Xu, H. Dai, P. Tu, Z. Li and X.M. Gao [2004] A study on the immune receptors for polysaccharides from the roots of Astragalus membranaceus: A Chinese medicinal herb Biochem. Biophys. Res. Comm. 320 (4), 1103–1111. Medline, ISI, Google Scholar
    • Shen, H.H., K. Wang, W. Li, Y.H. Ying, G.X. Gao, X.B. Li and H.Q. Huang [2008] Astragalus membranaceus prevents airway hyperreactivity in mice related to Th2 response inhibition J. Ethnopharmacol. 116 (2), 363–369. Medline, ISI, Google Scholar
    • Shimizu, N., M. Tomoda, M. Kanari and R. Gonda [1991] An acidic polysaccharide having activity on the reticuloendothelial system from the root of Astragalus mongholicus Chem. Pharm. Bull. (Tokyo) 39 (11), 2969–2972. Medline, ISI, Google Scholar
    • Shirataki, Y., M. Takao and A.S. Yoshid [1997] Antioxidative components isolated from the Astragalus membraneceus Bunge (astragali radix) Phytother. Res. 11, 603–605. ISI, Google Scholar
    • Sinclair, S. [1998] Chinese herbs: A clinical review of astragalus, ligusticum, and schizandrae Altern. Med. Rev. 3 (5), 338–344. Medline, Google Scholar
    • Song, C.Q., Z.R. Zheng, D. Liu and Z.B. Hu [1997a] Antimicrobial isoflavans from Astragalus membranaceus (Fisch.) Bunge Acta Botanica Sinica 39, 486–488. Google Scholar
    • Song, C.Q., Z.R. Zheng, D. Liu, Z.B. Hu and W.Y. Sheng [1997b] Isoflavones from Astragalus membranaceus Acta Botanica Sinica 39, 764–768. Google Scholar
    • Song, J.Z., S.F. Mo, Y.K. Yip, C.F. Qiao, Q.B. Han and H.X. Xu [2007] Development of microwave assisted extraction for the simultaneous determination of isoflavonoids and saponins in radix astragali by high performance liquid chromatography. J. Sep. Sci. 30 (6), 819–824. Medline, ISI, Google Scholar
    • Subarnas, A., Y. Oshima and H. Hikino [1991] New Constituents of Astragalus mongholicus Planta Med. 57 (6), 590. Medline, ISI, Google Scholar
    • Tang, J.L., B.Y. Liu and K.W. Ma [2008] Traditional Chinese medicine. Lancet 372 (9654), 1938–1940. Medline, ISI, Google Scholar
    • Tang, W. and G. Eisenbrand [1992] Chinese Drugs of Plant Origin. Beijing: Springer-Verlag, pp. 191–197. Google Scholar
    • Tang, W. and G. Eisenbrand [1992] Chinese Drugs of Plant Origin: Chemistry, Pharmacology, and Use in Traditional and Modern Medicine. Berlin: Springer-Verlag. Google Scholar
    • Tin, M.M., C.H. Cho, K. Chan, A.E. James and J.K. Ko [2007] Astragalus saponins induce growth inhibition and apoptosis in human colon cancer cells and tumor xenograft Carcinogenesis 28 (6), 1347–1355. Medline, ISI, Google Scholar
    • Toda, S. and Y. Shirataki [1999] Inhibitory effects of Astragali Radix, a crude drug in Oriental medicines, on lipid peroxidation and protein oxidative modification by copper J. Ethnopharmacol. 68 (68–3), 331–333. Medline, ISI, Google Scholar
    • Toda, S., Y. Yase and Y. Shirataki [2000] Inhibitory effects of astragali radix, crude drug in Oriental medicines on lipid peroxidation and protein oxidative modification of mouse brain homogenate by copper. Phytother. Res. 14 (4), 294–296. Medline, ISI, Google Scholar
    • Tu, L.H., D.R. Huang, R.Q. Zhang, Q. Shen, Y.Y Yu, Y.F. Hong and G.H. Li [1994] Regulatory action of Astragalus saponins and buzhong yiqi compound on synthesis of nicotinic acetylcholine receptor antibody in vitro for myasthenia gravis Chin. Med. J. (Engl.) 107 (4), 300–303. Medline, ISI, Google Scholar
    • Upton, R., G. Jolliffe and E. Williamson [2011] American Herbal Pharmacopoeia: Botanical Pharmacognosy–Microscopic Characterization of Botanical Medicines. Boca Raton: CRC Press. Google Scholar
    • Wagner, H.B., P.G. Xiao, J.M. Chen and G. Michler [1997] Radix Astragali (Huangqi): Chinese Drug Monographs and Analysis. Germany: Verlag, Wald, pp. 1–17. Google Scholar
    • Wang, D., W. Shen, Y. Tian, Z. Sun, C. Jiang and S. Yuan [1996] Protective effect of active components extracted from radix Astragali on human erythrocyte membrane damages caused by reactive oxygen species (in Chinese). Zhongguo Zhong Yao Za Zhi 21 (12), 746–748. Medline, Google Scholar
    • Wang, D.C. [1989] Influence of Astragalus membranaceus (AM) polysaccharide FB on immunologic function of human periphery blood lymphocyte (in Chinese) Zhonghua Zhong Liu Za Zhi 11 (3), 180–183. Medline, Google Scholar
    • Wang, P., Z. Zhang, X. Ma, Y. Huang, X. Liu, P. Tu and T. Tong [2003] HDTIC–1 and HDTIC–2: Two compounds extracted from astragali radix, delay replicative senescence of human diploid fibroblasts Mech. Ageing Dev. 124 (10–12), 1025–1034. Medline, ISI, Google Scholar
    • Wang, S.C., J.J. Shan, Z.T. Wang and Z. Hu [2006] Isolation and structural analysis of an acidic polysaccharide from Astragalus membranaceus (Fisch.) Bunge J. Integr. Plant Biol. 48, 1379–1384. ISI, Google Scholar
    • Wang, Y., K.K. Auyeung, X. Zhang and J.K. Ko [2014] AST modulates colon cancer development by regulating calpain-mediated glucose-regulated protein expression. BMC Comp. Altern. Med. 14 (1), 401. Medline, ISI, Google Scholar
    • Wang, Y., X.J. Qian, H.R. Hadley and B.H. Lau [1992] Phytochemicals potentiate interleukin-2 generated lymphokine-activated killer cell cytotoxicity against murine renal cell carcinoma. Mol. Biother. 4 (3), 143–146. Medline, Google Scholar
    • Wang, Y.P., X.Y. Li, C.Q. Song and Z.B. Hu [2002] Effect of astragaloside IV on T, B lymphocyte proliferation and peritoneal macrophage function in mice. Acta Pharmacol. Sin. 23 (3), 263–266. Medline, ISI, Google Scholar
    • Xiao, W., L. Han and B. Shi [2009] Isolation and purification of flavonoid glucosides from Radix Astragali by high-speed counter-current chromatography J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 877 (8–9), 697–702. Medline, ISI, Google Scholar
    • Yang, Z.G., H.X. Sun and W.H. Fang [2005] Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice Vaccine 23 (44), 5196–5203. Medline, ISI, Google Scholar
    • Yesilada, E., E. Bedir, I. Calis, Y. Takaishi and Y. Ohmoto [2005] Effects of triterpene saponins from Astragalus species on in vitro cytokine release J. Ethnopharmacol. 96 (1–2), 71–77. Medline, ISI, Google Scholar
    • Yin, F.G., Y.L. Liu, Y.L. Yin, X.F. Kong, R.L. Huang, T.J. Li, G.Y. Wu and Y. Hou [2009] Dietary supplementation with Astragalus polysaccharide enhances ileal digestibilities and serum concentrations of amino acids in early weaned piglets. Amino Acids 37 (2), 263–270. Medline, ISI, Google Scholar
    • Yin, J.Y., B.C.L. Chan, H. Yua, I.Y.K. Lau, X.Q. Han, S.W. Cheng and Q.B. Han [2012] Separation, structure characterization, conformation and immunomodulating effect of a hyperbranched heteroglycan from Radix Astragali. Carbohydr. Polym. 87, 667–675. Medline, ISI, Google Scholar
    • Yin, X., Y. Zhang, H. Wu, X. Zhu, X. Zheng, S. Jiang, H. Zhuo, J. Shen, L. Li and J. Qiu [2004] Protective effects of Astragalus saponin I on early stage of diabetic nephropathy in rats. J. Pharmacol. Sci. 95 (2), 256–266. Medline, ISI, Google Scholar
    • Yoshida, Y., M.Q. Wang, J.N. Liu, B.E. Shan and U. Yamashita [1997] Immunomodulating activity of Chinese medicinal herbs and Oldenlandia diffusa in particular Int. J. Immunopharmacol. 19 (7), 359–370. Medline, ISI, Google Scholar
    • Yu, D., Y. Duan, Y. Bao, C. Wei and L. An [2005] Isoflavonoids from Astragalus mongholicus protect PC12 cells from toxicity induced by L-glutamate J. Ethnopharmacol. 98 (1–2), 89–94. Medline, ISI, Google Scholar
    • Yu, D.H., Y.M. Bao, L.J. An and M. Yang [2009] Protection of PC12 cells against superoxide-induced damage by isoflavonoids from Astragalus mongholicus Biomed. Environ. Sci. 22 (1), 50–54. Medline, ISI, Google Scholar
    • Yu, Q.T., L.W. Qi, P. Yi, L. Li, J. Zhao and Z. Bi [2007] Determination of seventeen main flavonoids and saponins in the medicinal plant Huang-qi (Radix astragali) by HPLC-DAD-ELSD. J. Sep. Sci. 30 (9), 1292–1299. Medline, ISI, Google Scholar
    • Yu, S.Y., H.T. Ouyang, J.Y. Yang, X.L. Huang, T. Yang, J.P. Duan, J.P. Cheng, Y.X. Chen, Y.J. Yang and P. Qiong [2007] Subchronic toxicity studies of radix astragali extract in rats and dogs. J. Ethnopharmacol. 110, 352–355. Medline, ISI, Google Scholar
    • Yuan, W.L., H.Z. Chen, Y.Z. Yang, X.Y Yang, Y.S. Lin, P.Y. Jin and T.S. Zhou [1990] Effect of Astragalus membranaceus on electric activities of cultured rat beating heart cells infected with Coxsackie B-2 virus Chin. Med. J. (Engl.) 103 (3), 177–182. Medline, ISI, Google Scholar
    • Zee-Cheng, R.K. [1992] Shi-quan-da-bu-tang (ten significant tonic decoction), SQT: A potent Chinese biological response modifier in cancer immunotherapy, potentiation and detoxification of anticancer drugs. Methods Find. Exp. Clin. Pharmacol. 14 (9), 725–736. Medline, Google Scholar
    • Zhang, W.J., P. Hufnagl, B.R. Binder and J. Wojta [2003] Anti-inflammatory activity of astragaloside IV is mediated by inhibition of NF-κB activation and adhesion molecule expression. Thromb. Haemost. 90 (5), 904–914. Medline, ISI, Google Scholar
    • Zhang, Y.D., J.P. Shen, S.H. Zhu, D.K. Huang, Y. Ding and X.L. Zhang [1992] Effects of astragalus (ASI, SK) on experimental liver injury (in Chinese). Yao Xue Xue Bao 27 (6), 401–406. Medline, Google Scholar
    • Zhao, X.Z. [1992] Effects of Astragalus membranaceus and Tripterygium hypoglancum on natural killer cell activity of peripheral blood mononuclear in systemic lupus erythematosus (in Chinese) Zhongguo Zhong Xi Yi Jie He Za Zhi 12 (11), 669–671. Medline, Google Scholar
    • Zheng, H., Z. Dong and J. She [1997] Modern Study of Traditional Chinese Medicine. Beijing: Xue Yuan Press. Google Scholar