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Chemical characters and antioxidative properties of sulfated polysaccharides from Laminaria japonica

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Abstract

A low molecular weight sulfated polysaccharide (L-A) was prepared bymild acid hydrolysis of crude fucoidan (F-A) from Laminaria japonica. In comparison with F-A, L-A had a lower proportion of fucose residues,but a similar proportion of sulfate. The galactose content of both fractionswas relatively high, especially for L-A (57%). The antioxidant propertiesof the two polysaccharides were studied using two low-density lipoproteinoxidation systems. L-A had a stronger effect against low-density lipoproteinoxidation in both systems. F-A inhibited the AAPH-induced low-densitylipoprotein oxidation, but had little effect on the Cu++-inducedsystem due to its large molecular mass. The active sulfated fraction L-A,containing galactose, mannose and fucose (about 9: 2: 2) is reported herefor the first time.

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References

  • Albertini R, Rindi S, Passi A, Palladini G, Pallavicini G, De Luca G (1995) The effect of heparin on Cu2+-mediated oxidation of human low-density lipoproteins. FEBS Lett. 377: 240-242.

    Google Scholar 

  • Albertini R, Ramos P, Giessauf A, Passi A, De Luca G, Esterbauer H (1997) Chondroitin 4-sulphate exhibits inhibitory effect during Cu2+-mediated LDL oxidation. FEBS Lett. 403: 154-158.

    Google Scholar 

  • Anggadiredja J, Andyani R, Hayati, Muawanah (1997) Antioxidant activity of Sargassum polycystum (Phaeophyta) and Laurencia obtusa (Rhodophyta) from Seribu Islands. J. appl. Phycol. 9: 477-479.

    Google Scholar 

  • Bitter T, Muir HM (1962) A modified uronic acid carbazole reaction. Anal. Chem. 28: 350-356.

    Google Scholar 

  • Buege JA, Aust SD (1978) Microsomal lipid peroxidation. Meth. Enzy. 52: 302-310.

    Google Scholar 

  • Chevolot L, Foucault A, Chaubet F, Kervarec N, Sinquin C, Fisher AM, Boisson-Vidal C (1999) Further data on the structure of brown seaweed fucans: relationships with anticoagulant activity. Carbohydr. Res. 319: 154-165.

    Google Scholar 

  • Chizhov AO, Dell A, Morris HR, Haslam SM, McDowell RA, Shashkov AS, Nifant'ev NE, Khatuntseva EA, Usov AI (1999) A study of fucoidan from brown seaweed Chorda filum. Carbohydr. Res. 320: 108-119.

    Google Scholar 

  • Church FC, Meade JB, Treanor RE, Whinna HC (1989) Antithrombin activity of fucoidan. J. biol. Chem. 264: 3618-3623.

    Google Scholar 

  • Darrell G, Terry LS, Richard WB (1978) Structural features of a novel glucuronic galactifucan from Ascophyllum nodosum. Carbohydr. Res. 66: 167-171.

    Google Scholar 

  • Dodgson KS, Price RG (1962) A note on the determination of the ester sulfate content of sulfated polysaccharides. Biochem. J. 84: 106-110.

    Google Scholar 

  • Ito H, Sugiura M (1976) Antitumor polysaccharide fraction from Sargassum thunbergii. Chem. Pharm. Bull. 24: 1114-1115.

    Google Scholar 

  • Li TL, Wu CX, Zhang YX (1982) Studies of gas chromatographic analysis of saccharides and alditols ®: some improvements in analysis of acetylated aldononitriles by gas chromatography. Chinese J. anal. Chem. 10: 272-276.

    Google Scholar 

  • Liu XH, Zhang YS (1993) Fraction, purification and determination of the structure of fucoidan in Laminaria japonica Aresch. J. Liaoning Normal University (Natural Science) 16: 305-307.

    Google Scholar 

  • Manish SP, Sorgio O, Townsend B, et al. (1993) A revised structure of fucoidan may explain some of its biological activities. J. Biol. Chem.268: 21770-21776.

    Google Scholar 

  • Nishino T, Yokoyama G, Dobashi K, Fujihara M, Nagumo T (1989) Isolation, purification of fucose-containing sulfated polysaccharides from the brown seaweed Ecklonia kurome and their blood-anticoagulant activities. Carbohydr. Res. 186: 119-129.

    Google Scholar 

  • Nishino T, Aizu Y, Magamo T (1991) The relationship between the molecular weight and the anticoagulant activity of two types of fucan sulfates from the brown seaweed Ecklonia kurome. Agric. biol. Chem. 55: 791-796.

    Google Scholar 

  • Nishino T, Nagumo T, Kiyohara H, Yamada H (1991) Structural characterization of a new anticoagulant fucan sulfate from the brown seaweed Ecklonia kurome. Carbohydr. Res. 211: 77-90.

    Google Scholar 

  • Nishino T, Nagumo T (1991) The sulfate-content dependence of the anticoagulant activity of a fucan sulfate from the brown seaweedEcklonia kurome. Carbohydr. Res. 214: 193-197.

    Google Scholar 

  • Omata M, Matsui N, Inomata N, Ohno T (1997) Protective effects of polysaccharide fucoidan on myocardial ischemia-reperfusion injury in rats. J. Card. Pharmacol. 30: 717-724.

    Google Scholar 

  • Patanker MS, Oehninger S, Barnett T, Williams RL, Clark GF (1993) A revised structure of fucoidan may explain some of its biological activities. J. biol. Chem. 268: 21770-21776.

    Google Scholar 

  • Puhl H, Waeg G, Esterbauer H (1994) Methods to determine oxidation of low-density lipoproteins. Meth. Enzy. 233: 425-441.

    Google Scholar 

  • Reissing JL, Strominger LJ, Leloir FL (1955) A modified calorimetric method for the estimation of N-acetylamino sugars. J. Biol. Chem. 217: 959.

    Google Scholar 

  • Sato K, Niki E, Shimasaki H (1990) Free radical-mediated chain oxidation of low-density lipoprotein and its synergistic inhibition by vitamin E and vitamin C. Arch. Biochem. Biophys. 279: 402-405.

    Google Scholar 

  • Steinbrecher UP, Witaum JL, Parthasarathy S, Steinberg D (1987) Decrease in reactive amino groups during oxidation or endothelial mediated catabolism. Arteriosclerosis 7: 135-143.

    Google Scholar 

  • Soeda S, Ishida S, Shimeno H, Nagamastsu A (1994) Inhibitory effects of oversulfated fucoidan on invasion through reconstituted basement membrane by murine lewis lung carcinoma. Jpn. J. Cancer Res. 85: 1144-115</del>50.

    Google Scholar 

  • Taichi U, Katsuko A, Takashi M (1980) Isolation of highly purified fucoidan from Eisenia bicyclis. Agric. biol. Chem. 44: 1965-1966.

    Google Scholar 

  • Tutour BL, Benslimane F, Gouleau MP, Gouygou JP, Saadan B, Quemeneur F (1998) Antioxidant and pro-oxidant activities of the brown algae, Laminaria digitata, Himanthalia elongata, Fucus vesiculosus, Fucus serratus and Ascophyllum nodosum. J. appl. Phycol. 10: 121-129.

    Google Scholar 

  • Volpi N, Tarugi P (1999) Influence of chondroitin sulfate charge density, sulfate group position, and molecular mass on Cu2+-mediated oxidation of human low-density lipoproteins: effect of normal human plasma-derived chondroitin sulfate. J. Biochem. 125: 297-304.

    Google Scholar 

  • Xue CH, Yu GL, Hirata T, Terao J, Lin H (1998) Antioxidative activities of several marine polysaccharides evaluated in a phosphatidylcholine-liposomal suspension and organic solvents. Biosci. Biotech. Biochem. 62: 206-209.

    Google Scholar 

  • Yamamoto I, Nagumo T, Takahashi M, Fujihara M, Suzuki Y, Iizima N (1981) Antitumor effects of seaweed. Antitumor effect of an extract from Sargassum kjellmanianum. Jpn. J. exp. Med. 51: 187-189.

    Google Scholar 

  • Yamamoto I, Takahashi M, Suzuki T, Seino H, Mori H (1984) Antitumor effects of seaweed. Enhancement of antitumor activity by sulfation of a crude fucoidan from Sargassum kjellmanianum. Jpn. J. exp. Med. 54: 143-151.

    Google Scholar 

  • Zhang LH, Liu BW (1989) Separation of human serum lipoproteins by one-step ultracentrifugation. Progr. Biochem. Biophys. 21: 257-260.

    Google Scholar 

  • Zhuang C, Iton H, Mizuno T, Ito H (1995) Antitumor active fucoidan from the brown seaweed, Umitoranoo(Sargassum thunbergii). Biosci. Biotech. Biochem. 59: 563-567.

    Google Scholar 

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Authors and Affiliations

  1. Department of Food Science and Technology, Ocean University of Qingdao, No. 5, Yu Shan Road, Qingdao, Shandong Province, P.R. China, 266003

    Chang-Hu Xue, Yu Fang, Hong Lin, Lei Chen, Zhao-Jie Li, Deng Deng & Chong-Xiao Lu

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  1. Chang-Hu Xue
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  2. Yu Fang
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  3. Hong Lin
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Xue, CH., Fang, Y., Lin, H. et al. Chemical characters and antioxidative properties of sulfated polysaccharides from Laminaria japonica . Journal of Applied Phycology 13, 67–70 (2001). https://doi.org/10.1023/A:1008103611522

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