Journal of Food Science

Volume 77, Issue 9 p. C975-C982

Optimization of Ultrasonic-Assisted Extraction of Total Saponins from Eclipta prostrasta L. Using Response Surface Methodology

Ting Hu,

Ting Hu

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Yan-Yun Guo,

Yan-Yun Guo

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Qin-Fan Zhou,

Qin-Fan Zhou

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Xian-Ke Zhong,

Xian-Ke Zhong

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Liang Zhu,

Liang Zhu

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Jin-Hua Piao,

Jin-Hua Piao

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Jian Chen,

Jian Chen

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Jian-Guo Jiang,

Jian-Guo Jiang

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Ting Hu,

Ting Hu

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Yan-Yun Guo,

Yan-Yun Guo

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Qin-Fan Zhou,

Qin-Fan Zhou

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Xian-Ke Zhong,

Xian-Ke Zhong

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Liang Zhu,

Liang Zhu

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Jin-Hua Piao,

Jin-Hua Piao

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Jian Chen,

Jian Chen

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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Jian-Guo Jiang,

Jian-Guo Jiang

Authors are with the College of Food and Bioengineering, South China Univ. of Technology, Guangzhou, 510640, China. Direct inquiries to author Jiang (E-mail: [email protected] ).

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First published: 17 August 2012

https://doi.org/10.1111/j.1750-3841.2012.02869.x

Citations: 46

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Abstract

Abstract: Eclipta prostrasta L. is a traditional Chinese medicine herb, which is rich in saponins and has strong antiviral and antitumor activities. An ultrasonic-assisted extraction (UAE) technique was developed for the fast extraction of saponins from E. prostrasta. The content of total saponins in E. prostrasta was determined using UV/vis spectrophotometric methods. Several influential parameters like ethanol concentration, extraction time, temperature, and liquid/solid ratio were investigated for the optimization of the extraction using single factor and Box-Behnken experimental designs. Extraction conditions were optimized for maximum yield of total saponins in E. prostrasta using response surface methodology (RSM) with 4 independent variables at 3 levels of each variable. Results showed that the optimization conditions for saponins extraction were: ethanol concentration 70%, extraction time 3 h, temperature 70 °C, and liquid/solid ratio 14:1. Corresponding saponins content was 2.096%. The mathematical model developed was found to fit well with the experimental data.

Practical Application: Although there are wider applications of Eclipta prostrasta L. as a functional food or traditional medicine due to its various bioactivities, these properties are limited by its crude extracts. Total saponins are the main active ingredient of E. prostrasta. This research has optimized the extraction conditions of total saponins from E. prostrasta, which will provide useful reference information for further studies, and offer related industries with helpful guidance in practice.

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Volume77, Issue9

September 2012

Pages C975-C982

Optimization of Ultrasonic-Assisted Extraction of Total Saponins from Eclipta prostrasta L. Using Response Surface Methodology

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Optimization of Ultrasonic-Assisted Extraction of Total Saponins from Eclipta prostrasta L. Using Response Surface Methodology

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