Abstract
The effect of fucoidan (1.3; 1.4-α-L-fucan), a sulfated polysaccharide from the brown alga Fucus evanescens on the formation of specific granular and tubular inclusions induced by tobacco mosaic virus (TMV) and consisted presumably of the virus-coded protein components of the viral replicase was investigated in the TMV-infected leaves of tobacco (Nicotiana tabacum L.). In four days after inoculation of the leaves with a TMV preparation (1 mg/ml), the signature of infection in a presence of fucoidan (1 mg/ml) was a preferential formation of intracellular granular inclusions, which were related to early stages of the virus reproduction. When infected leaves were not treated with fucoidan, their cells contained mainly tubular inclusions, which were presumably formed from the granular ones on the last stages of the infection process. These observations demonstrated that fucoidan delayed the development of the TMV-induced infection.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- TMV:
-
tobacco mosaic virus
References
Lakhmatova, I.T., Induction of Plant Resistance to Viruses Using Biologically Active Substances (Immunization), S.-kh. Biol., 1992, no. 3, pp. 13–22.
Lyon, G.D., Reglinski, T., and Newton, A.C., Novel Disease Control Compounds: The Potential to “Immunize” Plants against Infection, Plant Pathol., 1995, vol. 44, pp. 407–427.
Reunov, A.V., Virusnyi patogenez i zashchitnye mekhanizmy rastenii (Viral Pathogenesis and Protective Mechanisms in Plants), Vladivostok: Dal’nauka, 1999.
Kopp, M., Rouster, J., Fritig, B., Darvill, A., and Albersheim, P., Host-Pathogen Interactions. XXXII. A Fungal Glucan Preparation Protects Nicotianae against Infection by Viruses, Plant Physiol., 1989, vol. 90, pp. 208–216.
Zinnen, T.M., Heinkel, C.M., Hudspeth, M.E.S., and Meganathan, R., The Role of Cytoplasmic Mycolaminaran in Inhibiting Initial Viral Infection of Certain Nicotiana Species, Phytopathology, 1991, vol. 81, pp. 426–428.
Heinkel, C.M., Hudspeth, M.E.S., Meganathan, R., and Zinnen, M., Further Characterization of Mycolaminaran-Induced Resistance. Temperature Sensitivity against Tobacco Mosaic Virus and Function against Cauliflower Mosaic Virus and Tomato Spotted Wilt Virus, Phytopathology, 1992, vol. 82, pp. 637–641.
Rouhier, P., Kopp, M., Begot, V., Bruneteau, M., and Fritig, B., Structural Features of Fungal β-D-Glucans for the Efficient Inhibition of the Initiation of Virus Infection on Nicotiana tabacum, Phytochemistry, 1995, vol. 39, pp. 57–62.
Reunov, A.V., Lapshina, L.A., Nagorskaya, V.P., and Elyakova, L.A., Effect of 1,3;1,6-β-D-Glucan on Infection of Detached Tobacco Leaves with Tobacco Mosaic Virus, J. Phytopathol., 1996, vol. 144, pp. 247–249.
Reunov, A.V., Lapshina, L.A., Nagorskaya, V.P., and Elyakova, L.A., The Inhibitory Effect of 1,3;1,6-β-D-Glucan on Potato Virus X Infection of Gomphrena and Datura Leaves, Fiziol. Rast. (Moscow), 2000, vol. 47, pp. 240–243 (Russ. J. Plant Physiol., Engl. Transl., pp. 211–215).
Stübler, D. and Buchenauer, H., Antiviral Activity of the Glucan Lichenan (Poly-β{1 → 3, 1 → 4}-D-Anhydroglucose): 1. Biological Activity in Tobacco Plants, J. Phytopathol., 1996, vol. 144, pp. 37–43.
Stübler, D. and Buchenauer, H., Antiviral Activity of the Glucan Lichenan (Poly-β{1 → 3, 1 → 4}-D-Anhydroglucose): 2. Studies on the Mode of Action, J. Phytopathol., 1996, vol. 144, pp. 45–52.
Pospieszny, H., Chirkov, S., and Atabekov, J., Induction of Antiviral Resistance in Plant by Chitosan, Plant Sci., 1991, vol. 79, pp. 63–68.
Chirkov, S.N., Antiviral Activity of Chitosan, Prikl. Biokhim. Mikrobiol., 2002, vol. 38, pp. 5–13.
Šubíková, V., Slováková, I., and Farkaš, V., Inhibition of Tobacco Necrosis Virus Infection by Xyloglucan Fragments, Z. Pflanzenkrankh. Pflanzensch., 1994, vol. 101, pp. 128–131.
Slováková, L., Lišková, D., Capek, P., Kubačková, M., Kákoniová, D., and Karácsonyi, Š., Defence Responses against TMV Infection Induced by Galactoglucomannan-Derived Oligosaccharides in Cucumber Cells, Eur. J. Plant Pathol., 2000, vol. 106, pp. 543–553.
Reunov, A., Nagorskaya, V., Lapshina, L., Yermak, I., and Barabanova, A., Effect of κ/β-Carragenan from Red Alga Tichocarpus crinitus (Tichocarpaceae) on Infection of Detached Tobacco Leaves with Tobacco Mosaic Virus, J. Plant Diseas. Protect., 2004, vol. 111, pp. 165–172.
Baba, M., Snoeck, R., Pauwels, R., and de Clercq, E., Sulfated Polysaccharides Are Potent and Selective Inhibitors of Various Enveloped Viruses, Including Herpes Simplex Virus, Cytomegalovirus, Vesicular Stomatitis Virus, and Human Immunodeficiency Virus, Antimicrob. Agents Chemother., 1988, vol. 32, pp. 1742–1745.
McClure, M.O., Moore, J.-P., Blanc, D.F., Scotting, P., Cook, G.M.W., Keynes, R.J., Weber, J., Davies, D., and Weiss, R.A., Investigations into the Mechanism by Which Sulfated Polysaccharides Inhibit HIV Infection In Vitro, AIDS Res. Human Retrovir., 1992, vol. 8, pp. 19–26.
Lapshina, L.A., Reunov, A.V., Nagorskaya, V.P., Zvyagintseva, T.N., and Shevchenko, N.M., Inhibitory Effect of Fucoidan from Brown Alga Fucus evanescens on the Spread of Infection Induced by Tobacco Mosaic Virus in Tobacco Leaves of Two Cultivars, Fiziol. Rast. (Moscow), 2006, vol. 53, pp. 274–279 (Russ. J. Plant Physiol., Engl. Transl., pp. 246–251).
Saito, T., Hosokawa, D., Meshi, T., and Okada, Y., Immunocytochemical Localization of the 130 K and 180 K Proteins (Putative Replicate Components) of Tobacco Mosaic Virus, Virology, 1987, vol. 160, pp. 477–481.
Kusaikin, M.I., Chizhov, A.O., Alekseeva, S.A., Bakunina, I.Yu., Nedashkovskaya, O.I., Sova, V.V., and Zvyagintseva, T.N., Comparative Investigation of Fucoidanase Specificity in Marine Microorganisms and Invertebrates, Dokl. Akad. Nauk, 2004, vol. 396, pp. 1–3.
Otsuki, Y., Takebe, I., Onho, T., Fukuda, M., and Okada, Y., Reconstitution of Tobacco Mosaic Virus Rods Occurs Bidirectionally from an Internal Initiation Region: Demonstration by Electron Microscopic Serology, Proc. Natl. Acad. Sci. USA, 1977, vol. 74, pp. 1913–1917.
Kiseleva, E.V., Shilov, A.G., and Khristolyubova, N.B., Methods for Estimation of Basic Stereological Parameters, Primenenie stereologicheskikh metodov v tsitologii (Using of Stereological Methods in Cytology), Gruzdev, A.D., Ed., Novosibirsk: Inst. Tsitol. Genet., 1974, pp. 33–53.
Esau, K., Viruses in Plant Hosts. Form, Distribution and Pathologic Effects, Madison: Wisconsin Univ., 1968.
Hills, G.J., Plaskitt, K.A., Young, N.D., Drenigan, D.D., Watts, J.W., Wilson, T.M.W., and Zaitlin, M., Immunogold Localization of the Intracellular Sites of Structural and Nonstructural Tobacco Mosaic Virus Proteins, Virology, 1987, vol. 161, pp. 488–496.
Reunov, A.V., Polyakova, A.M., and Reifman, V.G., Ultrastructural Characteristics of Viral Pathogenesis in Tissues of Young Tobacco Leaves Systemically Infected by TMV, Fiziol. Rast. (Moscow), 1983, vol. 30, pp. 115–122 (Sov. Plant Physiol., Engl. Transl.).
Vasil’ev, A.E. and Gamalei, Yu.V., Protein Crystals in Plant Cells, Tsitologiya, 1975, vol. 27, pp. 371–389.
Author information
Authors and Affiliations
Additional information
Original Russian Text © L.A. Lapshina, A.V. Reunov, V.P. Nagorskaya, T.N. Zvyagintseva, N.M. Shevchenko, 2007, published in Fiziologiya Rastenii, 2007, Vol. 54, No. 1, pp. 127–130.
Rights and permissions
About this article
Cite this article
Lapshina, L.A., Reunov, A.V., Nagorskaya, V.P. et al. Effect of fucoidan from brown alga Fucus evanescens on a formation of TMV-specific inclusions in the cells of tobacco leaves. Russ J Plant Physiol 54, 111–114 (2007). https://doi.org/10.1134/S1021443707010165
Received:
Issue Date:
DOI: https://doi.org/10.1134/S1021443707010165