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Qualitative and Semiquantitative Analysis of Fecal Bifidobacterium Species in Centenarians Living in Bama, Guangxi, China

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Abstract

Centenarians constitute a significant subpopulation in the Bama County of Guangxi province in China. The beneficial effects of intestinal microbiota, especially bifidobacteria of centenarians, have been widely accepted; however, knowledge about Bifidobacterium species in centenarians is not adequate. The aim of this study was to investigate the quantity and prevalence of fecal Bifidobacterium in healthy longevous individuals. Fecal samples from eight centenarians from Bama (aged 100 to 108 years), eight younger elderlies from Bama (aged 80 to 99 years), and eight younger elderlies from Nanning (aged 80 to 99 years) were analyzed using denaturing gradient gel electrophoresis, species-specific clone library, and quantitative polymerase chain reaction technology (qPCR). A total of eight different Bifidobacterium species were detected. B. dentium, B. longum, B. thermophilum, B. pseudocatenulatum/B. catenulatum, and B. adolescentis were common in fecal of centenarians and young elderly. B. minimum, B. saecularmay/B. pullorum/B. gallinarum, and B. mongoliense were found in centenarians but were absent in the younger elderlies. In addition, Bifidobacterium species found in centenarians were different from those found in Bama young elderly and Nanning young elderly, and the principal differences were the significant increase in the population of B. longum (P < 0.05) and B. dentium (P < 0.05) and the reduction in the frequency of B. adolescentis (P < 0.05), respectively. Centenarians tend to have more complex fecal Bifidobacterium species than young elderlies from different regions.

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Acknowledgments

The present work was supported by the grants from the National Natural Science Foundation of China (No. 31371762), the “Bagui Scholars Distinguished Professor” Special Project and Innovation Project of Guangxi Graduate Education (No.YCBZ2014019). We thank Dr. Bo Wu (Guangxi University) and Dr. Peihong Shen (Guangxi University) for providing the experiment guidance for denaturing gradient gel electrophoresis (DGGE). We have no conflicts of interest to declare.

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Authors and Affiliations

  1. College of Light Industry and Food Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, People’s Republic of China

    Fang Wang, Guohong Huang, Da Cai, Xiaolin Liang, Ting Yu, Haiyan Su, Jidong Liu, Mouming Zhao & Quanyang Li

  2. Food and Biological Technology Department, Guangxi Polytechnic, Ming Yang Industrial Park Jiangnan District, Nanning, 530226, People’s Republic of China

    Guohong Huang

  3. College of Life Sciences, Tianjin Normal University, Tianjin, 300387, People’s Republic of China

    Danlei Li

  4. College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, 530004, People’s Republic of China

    Peihong Shen

  5. Broad Technology Labs, Genome Sequencing and Analysis Program, Broad Institute, Boston, 02141, USA

    Hongcang Gu

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  1. Fang Wang
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Correspondence to Mouming Zhao or Quanyang Li.

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Wang, F., Huang, G., Cai, D. et al. Qualitative and Semiquantitative Analysis of Fecal Bifidobacterium Species in Centenarians Living in Bama, Guangxi, China. Curr Microbiol 71, 143–149 (2015). https://doi.org/10.1007/s00284-015-0804-z

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