Beta 1-6 branching of N-linked carbohydrate is associated with K-ras mutation in human colon carcinoma cell lines

Biochem Biophys Res Commun. 1995 Jul 26;212(3):758-66. doi: 10.1006/bbrc.1995.2034.

Abstract

Here the K-ras genotypes of nine colon carcinoma cell lines are compared to the protein glycosylation patterns found in these cells. By a variety of methodologies utilizing lectins to probe carbohydrate structure, we find evidence that five out of six cell lines having K-ras mutations have elevated amounts of beta 1-6 branching at the trimannosyl core of N-linked carbohydrate. None of the three K-ras wild type cell lines assayed have evidence of elevated beta 1-6 branching. In five out of five cell lines examined, the amount of beta 1-6 branching correlates with the extent of cellular ras-GTP elevation and supports the hypothesis that expression of beta 1-6 branching in colon carcinoma cell lines is quantitatively linked to K-ras activation. These results are discussed in the context of the ras-signalling pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Carbohydrates / chemistry*
  • Carbohydrates / genetics*
  • Cell Membrane / metabolism
  • Colonic Neoplasms / chemistry*
  • Colonic Neoplasms / genetics*
  • Colonic Neoplasms / metabolism
  • Genes, ras*
  • Genotype
  • Glycoproteins / chemistry
  • Glycoproteins / genetics
  • Glycosylation
  • Guanosine Triphosphate / metabolism
  • Humans
  • Lectins / metabolism
  • Molecular Sequence Data
  • Mutation*
  • Neoplasm Proteins / chemistry
  • Neoplasm Proteins / genetics
  • Phenotype
  • Tumor Cells, Cultured

Substances

  • Carbohydrates
  • Glycoproteins
  • Lectins
  • Neoplasm Proteins
  • Guanosine Triphosphate