Abstract
Taxomyces andreanae, a fungal endophyte, was isolated from the phloem (inner bark) of the Pacific yew, Taxus brevifolia. The fungus is hyphomyceteous and, when grown in a semi-synthetic liquid medium, produced taxol and related compounds. Taxol was identified by mass spectrometry, chromatography, and reactivity with monoclonal antibodies specific for taxol. Both [1-14C]acetic acid and L-[U-14C]phenylalanine served as precursors of [14C]taxol in fungal cultures. No taxol was detected in zero-time cultures or in the small agar plugs used to inoculate the culture flasks.
Publication types
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, Non-P.H.S.
- Comment
MeSH terms
- Antibodies, Monoclonal
- Bridged Bicyclo Compounds / metabolism*
- Bridged-Ring Compounds*
- Chromatography, Thin Layer
- Culture Media
- Humans
- Immunoenzyme Techniques
- KB Cells
- Mitosporic Fungi / metabolism*
- Paclitaxel / analysis
- Paclitaxel / biosynthesis*
- Paclitaxel / chemistry
- Paclitaxel / pharmacology
- Spectrometry, Mass, Fast Atom Bombardment
- Taxoids*
- Trees / microbiology*
Substances
- Antibodies, Monoclonal
- Bridged Bicyclo Compounds
- Bridged-Ring Compounds
- Culture Media
- Taxoids
- taxane
- Paclitaxel