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HuR facilitates cancer stemness of lung cancer cells via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis

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Abstract

Objectives

To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer.

Results

HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung cancer cells. Importantly, HuR and CDK3 expressions were increased in lung cancer tissues compared with normal adjacent tissues, and positively correlated. Mechanistically, HuR directly bound to CDK3, and increased CDK3 mRNA stability and expression. Additionally, miR-873 or miR-125a-3p attenuated the promotion of HuR on CDK3 expression and lung cancer stemness. Furthermore, HuR facilitated lung cancer stemness dependent on CDK3 expression. miR-873 or miR-125a-3p level was negatively correlated with HuR and CDK3 expression levels in lung cancer tissues.

Conclusions

HuR facilitates lung cancer stemness via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis.

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Supporting information

Supplementary Table 1—PCR primer sequences.

Author information

Author notes

  1. Yu Zhang and Limin Yang have equally contributed to this work.

Authors and Affiliations

  1. Department of Respiratory and Critical Medicine, The First Affiliated Hospital of Soochow University, Suzhou, 215000, China

    Yu Zhang, Limin Yang, Chunhua Ling & Wei Heng

  2. Department of Respiratory Medicine, Wuxi No.9 People’s Hospital affiliated to Soochow University, Wuxi, 214000, China

    Yu Zhang

Authors
  1. Yu Zhang
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  2. Limin Yang
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  3. Chunhua Ling
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  4. Wei Heng
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Corresponding authors

Correspondence to Chunhua Ling or Wei Heng.

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The authors declare no conflict of interest.

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Zhang, Y., Yang, L., Ling, C. et al. HuR facilitates cancer stemness of lung cancer cells via regulating miR-873/CDK3 and miR-125a-3p/CDK3 axis. Biotechnol Lett 40, 623–631 (2018). https://doi.org/10.1007/s10529-018-2512-9

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  • DOI: https://doi.org/10.1007/s10529-018-2512-9

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