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A new HPLC–UV method for the quantification of terpenoids and antioxidant activity of commercial loquat leaf tea and preparation

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Abstract

A number of recent studies on the loquat (Eriobotrya japonica Lindl.) leaf have demonstrated that loquat leaf extracts exhibited a wide range of pharmacological activities including hypoglycemic, anti-inflammatory, anti-tumor, neuroprotective and enhancement of brain health and liver function in the in vitro and animal models. Consequently, loquat leaf teas and dietary supplements are produced for human consumption. The aim of the present study was to develop a simple HPLC–UV method that can be used to simultaneously quantitate six triterpene acids and two sesquiterpene glycosides in commercial loquat leaf preparations including tea, granule and syrup, and to evaluate their total phenolic content and antioxidant activity. Total concentration of terpenes in six commercial products varied from 0.8 µg/mL to 14.8 mg/g, total phenolic content from 0.9 to 45.6 mg gallic acid equivalent/g, and antioxidant activity from 6.4 to 607.0 µmol trolox equivalent/g. The antioxidant activity correlated to total phenolic content significantly. The present study provided much needed information on the quality and content variability, and the potential of these products for future scientific investigations in human health.

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Acknowledgements

This work was supported by the Hirshberg Foundation for Pancreatic Cancer Research. Han Lü and Jian Chen were supported by a scholarship from Jiangsu Government Scholarship for Overseas Studies. We thank Jennifer Carney, MA, MPH for editing the manuscript.

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Authors and Affiliations

  1. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, 210014, People’s Republic of China

    Han Lü & Jian Chen

  2. Center for Human Nutrition, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA

    Ru-Po Lee, Jianjun Huang, Zhaoping Li & Qing-Yi Lu

  3. UCLA Agi Hirshberg Center for Pancreatic Diseases, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA

    Vay-Liang W. Go

Authors
  1. Han Lü
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  2. Ru-Po Lee
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  5. Vay-Liang W. Go
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  6. Zhaoping Li
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Correspondence to Qing-Yi Lu.

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Lü, H., Lee, RP., Huang, J. et al. A new HPLC–UV method for the quantification of terpenoids and antioxidant activity of commercial loquat leaf tea and preparation. Food Measure 14, 1085–1091 (2020). https://doi.org/10.1007/s11694-019-00358-3

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  • DOI: https://doi.org/10.1007/s11694-019-00358-3

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