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Nicotinamide N-methyltransferase in Non-small Cell Lung Cancer: Promising Results for Targeted Anti-cancer Therapy

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Abstract

Lung cancer, predominantly non-small cell lung cancer (NSCLC), is currently the most common cause of malignancy-related death in the world. Despite advances in both detection and treatment, its incidence rate is still increasing. Therefore, effective strategies for early detection as well as molecular therapeutic targets are urgently needed. We focused on the enzyme nicotinamide N-methyltransferase (NNMT). NNMT expression levels were investigated in tumor, tumor-adjacent, and surrounding tissue samples of 25 patients with NSCLC by Real-Time PCR, Western blot analysis, and catalytic activity assay. NNMT enzyme activity in NSCLC was then correlated with clinicopathological characteristics. Results obtained showed NNMT upregulation (mRNA and protein) in tumor compared with both tumor-adjacent and surrounding tissue. Moreover, NSCLC displayed significantly higher activity levels than those determined in both tumor-adjacent and surrounding tissue. Interestingly, both tumor-adjacent and surrounding tissue samples of unfavorable cases (N+) seem to display higher activity levels than those of favorable NSCLCs (N0). The present work shows a marked increase of NNMT enzyme activity in NSCLC and suggests that normal-looking tissue of unfavorable cases seems to change toward cancer. Further studies may establish whether NNMT could represent a target for an effective anti-cancer therapy.

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Authors and Affiliations

  1. Sezione di Biochimica, Dipartimento di Scienze Cliniche Specialistiche e Odontostomatologiche, Università Politecnica delle Marche, Via Ranieri 65, 60131, Ancona, Italy

    Davide Sartini, Stefano Morganti, Elena Guidi, Rachela Giuliante, Valentina Pozzi & Monica Emanuelli

  2. Sezione di Anatomia Patologica e Istopatologia, Dipartimento di Scienze Biomediche e Sanità Pubblica, Università Politecnica delle Marche, Ancona, Italy

    Corrado Rubini & Antonio Zizzi

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  1. Davide Sartini
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  2. Stefano Morganti
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  3. Elena Guidi
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  4. Corrado Rubini
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  7. Valentina Pozzi
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  8. Monica Emanuelli
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Correspondence to Davide Sartini.

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Sartini, D., Morganti, S., Guidi, E. et al. Nicotinamide N-methyltransferase in Non-small Cell Lung Cancer: Promising Results for Targeted Anti-cancer Therapy. Cell Biochem Biophys 67, 865–873 (2013). https://doi.org/10.1007/s12013-013-9574-z

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  • DOI: https://doi.org/10.1007/s12013-013-9574-z

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