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Radiation protection of HepG2 cells by Podophyllum hexandrum Royale

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Abstract

Radioprotection by an aqueous extract of Podophyllum hexandrum (RP-1) was investigated in HepG2 cells by evaluating colony forming efficacy (CFE), redox status of mitochondria, reactive oxygen species (ROS), generation of nitric oxide (NO), peroxidation of lipids and intracellular glutathione. Lower concentrations of RP-1 (0.1 and 1 μg/ml) rendered maximum radioprotection when administered 1 or 2 h before irradiation. Higher concentrations (5 and 10 μg/ml) however were less effective when administered 1 or 2 h before irradiation, but were more effective with increased time intervals (4 or 8 h) between RP-1 administration and irradiation. RP-1 pre-treatment also significantly inhibited radiation-induced MTT reduction in a concentration and time-dependent manner by decreasing gamma radiation-induced leakage of electrons from electron transport chain. Pre-irradiation administration of RP-1 significantly reduced both ROS and NO generation and enhanced glutathione levels, thereby inhibiting lipid peroxidation.

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Authors and Affiliations

  1. Radiation Biology Division, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organization (DRDO), Delhi, India

    Damodar Gupta & Rajesh Arora

  2. Department of Microbiology, Chaudhary Charan Singh University, Meerut, India

    Amar Prakash Garg

  3. Radiation Biology Division, Institute of Nuclear Medicine and Allied Sciences, (DRDO), Brig. SK Majumdar Road, Delhi, 110054, India

    Harish Chandra Goel

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  1. Damodar Gupta
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  4. Harish Chandra Goel
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Gupta, D., Arora, R., Garg, A.P. et al. Radiation protection of HepG2 cells by Podophyllum hexandrum Royale. Mol Cell Biochem 250, 27–40 (2003). https://doi.org/10.1023/A:1024925612233

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