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Haploid Plants from Pollen Grains

J. P. Nitsch and C. NitschAuthors Info & Affiliations
Science
3 Jan 1969
Vol 163, Issue 3862
pp. 85-87
DOI: 10.1126/science.163.3862.85
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Abstract

A method is presented by which hundreds of haploid plants of various species of Nicotiana can be raised from pollen grains. Stamens should be excised when pollen grains have been individualized, but are still uninucleate and free of starch. When grown in vitro on a relatively simple medium, some pollen grains proliferate into embryo-like structures which develop in stages similar to those of zygotic embryos. The plantlets mature and flower profusely, but do not set seed.

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References

BOURGIN, J.P., PRODUCTION OF HAPLOIDS NICOTIANA FROM EXCISED STAMENS, ANNALES DE PHYSIOLOGIE VEGETALE 9: 377 (1967).
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CLAUSEN, R.E., Interspecific hybridization in nicotiana. X. Haploid and diploid merogony, AMERICAN NATURALIST 63: 279 (1929).
Google Scholar
HALPERIN, W, AMMONIUM REQUIREMENT FOR EMBRYOGENESIS IN VITRO, NATURE 205: 519 (1965).
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Kostoff, D., Zeitschrift Fur Zellforschung Und Mikroskopische Anatomie 9: 640 (1929).
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NAKATA, K, DIFFERENTIATION OF EMBRYOIDS FROM DEVELOPING GERM CELLS IN ANTHER CULTURE OF TOBACCO, JAPANESE JOURNAL OF GENETICS 43: 65 (1968).
Google Scholar
NITSCH, J.P., EXPERIMENTAL PRODUCTION OF ANDROGENESIS IN VARIOUS NICOTIANA, COMPTES RENDUS DES SEANCES DE LA SOCIETE DE BIOLOGIE ET DE SES FILIALES 162: 369 (1968).
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Information & Authors

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Published In

Science
Volume 163 | Issue 3862
3 January 1969

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© 1969.

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Published in print: 3 January 1969

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Authors

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J. P. Nitsch
Laboratoire de Physiologie Pluricellulaire, Centre National de la Recherche Scientifique, 91, Gif-sur-Yvette, France
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C. Nitsch
Laboratoire de Physiologie Pluricellulaire, Centre National de la Recherche Scientifique, 91, Gif-sur-Yvette, France
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  • J. P. Nitsch,
  • C. Nitsch
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Haploid Plants from Pollen Grains.Science163,85-87(1969).DOI:10.1126/science.163.3862.85

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Cited by

    • R. S. Chaleff,
    • T. B. Ray,
    Herbicide-Resistant Mutants from Tobacco Cell Cultures, Science, 223, 4641, (1148-1151), (1984)./doi/10.1126/science.223.4641.1148
    Abstract
    • Peter S. Carlson,
    Induction and Isolation of Auxotrophic Mutants in Somatic Cell Cultures of Nicotiana tabacum, Science, 168, 3930, (487-489), (1970)./doi/10.1126/science.168.3930.487
    Abstract

Citation information is sourced from Crossref Cited-by service.

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