An efficient temporary immersion system for micropropagation of hybrid hazelnut
Abstract
An efficient protocol for the micropropagation of hybrid hazelnut (Corylus L.) was developed using a temporary immersion system (TIS) for the proliferation of shoots in a liquid medium. Growth and proliferation of shoots as well as the length and number of nodes in developing shoots were significantly improved by combining two forms of chelated iron: ethylenediamine-N,N′-di-(ortho-hydroxyphenyl) acetic acid (FeEDDHA), and FeSO4/EDTA (FeEDTA). The use of TIS with optimized concentrations of FeEDTA and FeEDDHA significantly increased the number of shoots, shoot height, leaf area, and chlorophyll content in all of the four cultivars tested, compared with a semi-solid medium. Proliferated shoots developed roots on a semi-solid medium containing 2.5 μmol/L indole-3-butyric acid and rooted plantlets acclimatized in greenhouse conditions with 80% survival.
Résumé
Les auteurs ont développé un protocole efficace de micro-propagation de noisetier hybride (Corylus L.) qui utilise un système d’immersion temporaire (SIT) permettant de multiplier les pousses en milieu liquide. La croissance et la multiplication des pousses de même que la longueur et le nombre de nœuds présents sur les pousses en développement étaient significativement améliorés en combinant deux formes de fer chélaté, l’acide éthylènediamine-N,N′-di-(ortho-hydroxyphényl) acétique (FeEDDHA) et le FeSO4/EDTA. L’utilisation du SIT et de concentrations optimisées de FeEDTA et de FeEDDHA accroissait significativement le nombre de pousses, la hauteur des pousses, la surface foliaire et le contenu en chlorophylle des quatre cultivars testés, comparativement à un milieu semi-solide. Les pousses en prolifération développaient des racines sur un milieu semi-solide contenant 2,5 μmol/L d’IBA et les plantules enracinées s’acclimataient aux conditions de serre avec un taux de survie de 80 %. [Traduit par la Rédaction]
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Published In
Botany
Volume 94 • Number 1 • January 2016
Pages: 1 - 8
History
Received: 9 June 2015
Accepted: 20 October 2015
Accepted manuscript online: 26 October 2015
Version of record online: 26 October 2015
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© 2015.
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Jyoti Latawa, Mukund R. Shukla, and Praveen K. Saxena. 2016. An efficient temporary immersion system for micropropagation of hybrid hazelnut. Botany. 94(1): 1-8. https://doi.org/10.1139/cjb-2015-0111