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Metabolic analysis of elicited cell suspension cultures of Cannabis sativa L. by 1H-NMR spectroscopy

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Abstract

Cannabis sativa L. plants produce a diverse array of secondary metabolites. Cannabis cell cultures were treated with jasmonic acid (JA) and pectin as elicitors to evaluate their effect on metabolism from two cell lines using NMR spectroscopy and multivariate data analysis. According to principal component analysis (PCA) and partial least square-discriminant analysis (PLS-DA), the chloroform extract of the pectin-treated cultures were more different than control and JA-treated cultures; but in the methanol/water extract the metabolome of the JA-treated cells showed clear differences with control and pectin-treated cultures. Tyrosol, an antioxidant metabolite, was detected in cannabis cell cultures. The tyrosol content increased after eliciting with JA.

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Acknowledgements

I.J. Flores Sanchez received a partial grant from Consejo Nacional de Ciencia y Tecnologia (Mexico) and J. Peč received a grant from Ministry of Education of the Czech Republic (MSM 0021620822).

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Authors and Affiliations

  1. Section of Metabolomics, Pharmacognosy Department, Institute of Biology, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands

    Isvett Josefina Flores-Sanchez, Young Hae Choi & Robert Verpoorte

  2. Faculty of Pharmacy, Pharmacognosy Department, Charles University in Prague, Heyrovského 1203, 500 05, Hradec Králové, Czech Republic

    Jaroslav Peč

Authors
  1. Jaroslav Peč
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  2. Isvett Josefina Flores-Sanchez
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  3. Young Hae Choi
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  4. Robert Verpoorte
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Correspondence to Robert Verpoorte.

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Peč, J., Flores-Sanchez, I.J., Choi, Y.H. et al. Metabolic analysis of elicited cell suspension cultures of Cannabis sativa L. by 1H-NMR spectroscopy. Biotechnol Lett 32, 935–941 (2010). https://doi.org/10.1007/s10529-010-0225-9

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  • DOI: https://doi.org/10.1007/s10529-010-0225-9

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