Comparative proteomic and phosphoproteomic profiling of pancreatic adenocarcinoma cells treated with CB1 or CB2 agonists
Jessica Brandi
Proteomics and Mass Spectrometry Laboratory, Department of Biotechnology, University of Verona, Verona, Italy
These authors equally contributed to this work.
Search for more papers by this authorIlaria Dando
Biological Chemistry Section Department of Life and Reproduction Sciences, University of Verona, Verona, Italy
These authors equally contributed to this work.
Search for more papers by this authorMarta Palmieri
Biological Chemistry Section Department of Life and Reproduction Sciences, University of Verona, Verona, Italy
Search for more papers by this authorMassimo Donadelli
Biological Chemistry Section Department of Life and Reproduction Sciences, University of Verona, Verona, Italy
Search for more papers by this authorCorresponding Author
Daniela Cecconi
Proteomics and Mass Spectrometry Laboratory, Department of Biotechnology, University of Verona, Verona, Italy
Correspondence: Dr. Daniela Cecconi, Dipartimento di Biotecnologie, Laboratorio di Proteomica e Spettrometria di massa, Università degli Studi di Verona, Strada Le Grazie 15, 37134 Verona, Italy
E-mail:[email protected]
Fax: +39-045-8027929
Search for more papers by this authorJessica Brandi
Proteomics and Mass Spectrometry Laboratory, Department of Biotechnology, University of Verona, Verona, Italy
These authors equally contributed to this work.
Search for more papers by this authorIlaria Dando
Biological Chemistry Section Department of Life and Reproduction Sciences, University of Verona, Verona, Italy
These authors equally contributed to this work.
Search for more papers by this authorMarta Palmieri
Biological Chemistry Section Department of Life and Reproduction Sciences, University of Verona, Verona, Italy
Search for more papers by this authorMassimo Donadelli
Biological Chemistry Section Department of Life and Reproduction Sciences, University of Verona, Verona, Italy
Search for more papers by this authorCorresponding Author
Daniela Cecconi
Proteomics and Mass Spectrometry Laboratory, Department of Biotechnology, University of Verona, Verona, Italy
Correspondence: Dr. Daniela Cecconi, Dipartimento di Biotecnologie, Laboratorio di Proteomica e Spettrometria di massa, Università degli Studi di Verona, Strada Le Grazie 15, 37134 Verona, Italy
E-mail:[email protected]
Fax: +39-045-8027929
Search for more papers by this authorAbstract
The pancreatic adenocarcinoma cell line Panc1 was treated with cannabinoid receptor ligands (arachidonylcyclopropylamide or GW405833) in order to elucidate the molecular mechanism of their anticancer effect. A proteomic approach was used to analyze the protein and phosphoprotein profiles. Western blot and functional data mining were also employed in order to validate results, classify proteins, and explore their potential relationships. We demonstrated that the two cannabinoids act through a widely common mechanism involving up- and down-regulation of proteins related to energetic metabolism and cell growth regulation. Overall, the results reported might contribute to the development of a therapy based on cannabinoids for pancreatic adenocarcinoma.
Supporting Information
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| Filename | Description |
|---|---|
| elps4656-sup-0001-S1.rar2 MB | Supporting Information. |
| elps4656-sup-0002-Figure1.ppt1.3 MB | Figure S1. Two-dimensional maps of Panc1 cell line untreated or treated with 200 μM ACPA or GW405833 for 12 hours. Total proteome (Sypro Ruby stained) and phosphoproteome profiles (Pro-Q Diamond-stained) are shown in the upper and lower line respectively. |
| elps4656-sup-0003-Figure2.ppt1.3 MB | Figure S2. Master gel of the of protein and phoshoprotein profiles of Panc1 cells. The cross indicate spots with significantly different expression levels (p < 0.05) between untreated and ACPA or GW treated samples The spot numbers in the maps are the same as those used for the mass spectrometry identification shown in Supplemental Table 2. |
| elps4656-sup-0004-Table1.doc27 KB | Table S1. The different primary antibodies used for Western Blot analysis, with the corresponding quantity of protein loaded and Ab diluitions. |
| elps4656-sup-0005-Table2.doc249.5 KB | Table S2. Proteins (in black) and phosphoproteins (in blue) with modulated abundance identified by 2-DE and nano-HPLC-Chip MS/MS analysis. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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