ERK1/2 activity contributes to gemcitabine resistance in pancreatic cancer cells
Abstract
Objective
Methods
Results
Conclusions
Introduction
Materials and methods
Cell culture
Development of PANC-1GemRes
Determination of gemcitabine IC50
Cell proliferation and apoptosis analysis
Western blotting
Mouse tumour model
Statistical analyses
Results
Cell line | Gemcitabine | Gemcitabine + U0126 |
---|---|---|
BxPC-3 | 14.0 ± 2.1 nM | 7.9 ± 1.2 nM |
PANC-1 | 49.6 ± 5.4 nM | 19.7 ± 4.3 nMb |
PANC-1GemRes | 12.4 ± 1.8 µMa | 2.4 ± 0.5 µMb |
Treatment | Cell line | ||
---|---|---|---|
BxPC-3 | PANC-1 | PANC-1GemRes | |
Control | 1.12 ± 0.02 | 1.04 ± 0.03 | 1.16 ± 0.02 |
U0126 | 1.14 ± 0.02 | 1.13 ± 0.03 | 1.18 ± 0.04 |
Gemcitabine | 13.5 ± 1.46* | 5.14 ± 1.05 | 1.23 ± 0.06 |
U0126 + gemcitabine | 37.4 ± 6.3*** | 17.3 ± 2.54** | 19.32 ± 2.46** |
Parameter | Cell line | ||
---|---|---|---|
BxPC-3 | PANC-1 | PANC-1GemRes | |
Tumour volume, mm3 | |||
Control | 2540 ± 250 | 2350 ± 280 | 2120 ± 320 |
U0126 | 2430 ± 235 | 2286 ± 315 | 2216 ± 280 |
Gemcitabine | 1760 ± 85* | 2032 ± 178 | 2080 ± 260 |
U0126 + gemcitabine | 210 ± 35** | 1100 ± 65* | 1650 ± 120* |
Apoptosisa | |||
Control | 1.02 ± 0.08 | 1.83 ± 0.12 | 1.14 ± 0.12 |
U0126 | 1.08 ± 0.07 | 1.92 ± 0.12 | 1.24 ± 0.13 |
Gemcitabine | 4.46 ± 0.13 | 2.17 ± 0.15 | 2.15 ± 0.13 |
U0126 + gemcitabine | 14.30 ± 1.16*** | 11.20 ± 2.30*** | 9.70 ± 2.54** |
Discussion
Declaration of conflicting interest
Funding
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This article was published in Journal of International Medical Research.
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