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Anti-tumour immune effect of oral administration of Lactobacillus plantarum to CT26 tumour-bearing mice

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Abstract

Colorectal cancer (CRC) is one of the most prevalent forms of cancer that shows a high mortality and increasing incidence. There are numerous successful treatment options for CRC, including surgery, chemotherapy, radiotherapy and immunotherapy; however, their side effects and limitations are considerable. Probiotics may be an effective strategy for preventing and inhibiting tumour growth through stimulation of host innate and adaptive immunity. We investigated and compared potential anti-tumour immune responses induced by two isolated Lactobacillus strains, Lactobacillus plantarum A and Lactobacillus rhamnosus b, by pre-inoculating mice with lactobacilli for 14 days. Subsequently, subcutaneous and orthotopic intestinal tumours were generated in the pre-inoculated mice using CT26 murine adenocarcinoma cells and were assessed for response against the tumour. Our results indicated that oral administration with L. plantarum inhibited CT26 cell growth in BALB/c mice and prolonged the survival time of tumour-bearing mice compared with mice administered L. rhamnosus. L. plantarum produced protective immunity against the challenge with CT26 cells by increasing the effector functions of CD8+ and natural killer (NK) cell infiltration into tumour tissue, up-regulation of IFN-γ (but not IL-4 or IL-17) production, and promotion of Th1-type CD4+ T differentiation. Consequently, our results suggest that L. plantarum can enhance the anti-tumour immune response and delay tumour formation.

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Abbreviations

APC:

allophycocyanin

CFU:

colony forming unit

CRC:

colorectal cancer

DC:

dendritic cells

ELISA:

enzyme-linked immunosorbent assay

FITC:

fluorescein isothiocyanate

IFN:

interferon

IL:

interleukin

MRS:

deMan, Rogosa and Sharpe

NK:

natural killer cells

PE:

phycoerythrin

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31302061), the Ministry of Education University Doctoral Fund (20110061110005), and Chinese Special Fund for Agro-Scientific Research in the Public Interest (201303033).

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Authors and Affiliations

  1. College of Veterinary Medicine, Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun, 130062, China

    Jingtao Hu & Zhuang Ding

  2. College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, China

    Jingtao Hu, Chunfeng Wang, Liping Ye, Wentao Yang, Haibin Huang, Fei Meng & Shaohua Shi

  3. Guangxi Veterinary Research Institute, 51 You Ai Road, Nanning, Guangxi, 530001, China

    Fei Meng

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  1. Jingtao Hu
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  2. Chunfeng Wang
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  4. Wentao Yang
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  5. Haibin Huang
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  7. Shaohua Shi
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  8. Zhuang Ding
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Correspondence to Chunfeng Wang or Zhuang Ding.

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Corresponding editor: Murali Bashyam

[Hu J, Wang C, Ye L, Yang W, Huang H, Meng F, Shi S and Ding Z 2015 Anti-tumour immune effect of oral administration of Lactobacillus plantarum to CT26 tumour-bearing mice. J. Biosci. 40 1–11] DOI 10.1007/s12038-015-9518-4

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Hu, J., Wang, C., Ye, L. et al. Anti-tumour immune effect of oral administration of Lactobacillus plantarum to CT26 tumour-bearing mice. J Biosci 40, 269–279 (2015). https://doi.org/10.1007/s12038-015-9518-4

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