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Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model

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Abstract

Probiotics have been suggested as a prophylactic measure in colon cancer. The aim of this study was to investigate the impact of Lactobacillus salivarius Ren (Ren) in modulating colonic microbiota structure and colon cancer incidence in a rat model after injection with 1,2-dimethyl hydrazine (DMH). The results indicated that oral administration of Ren could effectively suppress DMH-induced colonic carcinogenesis. A significant decrease in cancer incidence (87.5% to 25%) was detected in rats fed with a dose of 5 × 1010 CFU/kg bodyweight per day. Using denaturing gradient gel electrophoresis and Real-time PCR combined with multivariate statistical methods, we demonstrated that injection with DMH significantly altered the rat gut microbiota, while Ren counteracted these DMH-induced adverse effects and promoted reversion of the gut microbiota close to the healthy state. Tvalue biplots followed by band sequencing identified 21 bacterial strains as critical variables affected by DMH and Ren. Injection of DMH significantly increased the amount of Ruminococcus species (sp.) and Clostridiales bacteria, as well as decreasing the Prevotella sp. Administration of Ren reduced the amount of Ruminococcus sp., Clostridiales bacteria, and Bacteroides dorei, and increased the amount of Prevotella. Real-time PCR results were consistent with the results derived by t-value biplots. These findings suggested that Ren is a potential agent for colon cancer prevention. In conclusion, the results in the present study suggest a potential therapeutic approach based on the modulation of intestinal microflora by probiotics may be beneficial in the prevention of colorectal carcinogenesis.

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Authors and Affiliations

  1. School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing, 100048, P. R. China

    Ming Zhang

  2. Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, P. R. China

    Xing Fan, Chengzhen Zhu, Jun Zhu & Fazheng Ren

  3. Academy of State Administration of Grain, Beijing, 100037, P. R. China

    Bing Fang

  4. Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing, 100048, China

    Ming Zhang

  5. Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing, 100083, P. R. China

    Xing Fan, Chengzhen Zhu, Jun Zhu & Fazheng Ren

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  1. Ming Zhang
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  2. Xing Fan
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  3. Bing Fang
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  4. Chengzhen Zhu
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  5. Jun Zhu
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  6. Fazheng Ren
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Correspondence to Fazheng Ren.

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These authors contributed equally to this work

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Zhang, M., Fan, X., Fang, B. et al. Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model. J Microbiol. 53, 398–405 (2015). https://doi.org/10.1007/s12275-015-5046-z

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  • DOI: https://doi.org/10.1007/s12275-015-5046-z

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