Cookies Notification

We use cookies to improve your website experience. To learn about our use of cookies and how you can manage your cookie settings, please see our Cookie Policy.
×

Comment on "Identifying spiders through DNA barcodes"

Publication: Canadian Journal of Zoology
March 2005

Abstract

R.D.H. Barrett and P.D.N Hebert have demonstrated that it is possible to identify members of a mostly local spider fauna using a short fragment of the mitochondrial gene coding for cytochrome c oxidase I. There are instances where DNA-based identification may be very useful, e.g., in identifying juvenile life stages of groups in which adults are required for morphological identification, or matching morphologically different sexes or life stages when those associations are unknown. DNA-based identification may be the easiest and most cost-effective way, or even the only feasible way, to address some of these questions. However, these are also the least challenging problems in taxonomy, and their solution is unlikely to relieve the "taxonomic impediment". Furthermore, to promote the utility of DNA barcoding as a global identification system, these authors must demonstrate that their approach works for distinguishing all the members of a speciose clade, wherever in the world they occur. Much of diversity occurs allopatrically and neither the study by R.D.H. Barrett and P.D.N. Hebert, nor any other presented to date, even begins to address the feasibility of DNA-based identification at this level of detail.

Résumé

R.D.H. Barrett et P.D.N. Hebert ont démontré qu'il était possible d'identifier les composantes d'une faune surtout locale d'araignées à partir d'un court segment type du gène mitochondrial de la cytochrome c oxydase I. Il y a des occasions où l'identification à partir de l'ADN peut s'avérer très utile, par exemple pour reconnaître les stades immatures chez les groupes où l'identification morphologique exige des spécimens adultes ou pour apparier des spécimens de sexe ou de stade différents qui ont des morphologies distinctes lorsque ces associations sont inconnues. L'identification à partir de l'ADN peut s'avérer être la méthode la plus facile et la moins coûteuse, et quelquefois la seule, pour résoudre ces questions. Cependant, il s'agit là de problèmes de taxonomie qui sont loin de poser un défi majeur et leur solution ne viendra vraisemblablement pas atténuer « l'obstacle taxonomique ». De plus, pour promouvoir l'utilisation des codes-barres d'ADN comme système global d'identification, les auteurs devront démonter que leur méthode permet de distinguer tous les membres d'un clade riche en espèces, où qu'ils se trouvent dans le monde. Une partie importante de la diversité se retrouve en situations d'allopatrie; ni l'étude de R.D.H. Barrett et de P.D.N. Hebert, ni aucune autre publiée jusqu'à maintenant, n'abordent, même de façon préliminaire, l'applicabilité de l'identification à partir de l'ADN à ce niveau de détails.[Traduit par la Rédaction]

Get full access to this article

View all available purchase options and get full access to this article.

Information & Authors

Information

Published In

cover image Canadian Journal of Zoology
Canadian Journal of Zoology
Volume 83Number 3March 2005
Pages: 498 - 504

History

Version of record online: 15 February 2011

Permissions

Request permissions for this article.

Authors

Affiliations

Metrics & Citations

Metrics

Other Metrics

Citations

Cite As

Export Citations

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

Cited by

1. The Case for a Nuclear Barcode: Using the CAD CPS Region for Species and Genus Level Discrimination in Beetles
2. What’s left in the tank? Identification of non-ascribed aquarium’s coral collections with DNA barcodes as part of an integrated diagnostic approach
3. Plant DNA Barcoding
4. South Africa’s contribution of insect records on the BOLD system
5. Plant DNA Barcoding
6. The importance of molecular characters when morphological variability hinders diagnosability: systematics of the moon jellyfish genus Aurelia (Cnidaria: Scyphozoa)
7. The Essential Role of Taxonomic Expertise in the Creation of DNA Databases for the Identification and Delimitation of Southeast Asian Ambrosia Beetle Species (Curculionidae: Scolytinae: Xyleborini)
8. Implications and Utility of DNA Barcoding
9. Systematic revision of Mexican threatened tarantulas Brachypelma (Araneae: Theraphosidae: Theraphosinae), with a description of a new genus, and implications on the conservation
10. Review and Interpretation of Trends in DNA Barcoding
11. Leeches from Mexico City, remnants of the ancient lake
12. DNA barcoding as a tool to facilitate the taxonomy of hermit crabs (Decapoda: Anomura: Paguroidea)
13.
14. The Science of Insect Taxonomy: Prospects and Needs
15. The complete mitochondrial genome of Thitarodes sejilaensis (Lepidoptera: Hepialidae), a host insect of Ophiocordyceps sinensis and its implication in taxonomic revision of Hepialus adopted in China
16. Systematic revision of Brachypelma red-kneed tarantulas (Araneae : Theraphosidae), and the use of DNA barcodes to assist in the identification and conservation of CITES-listed species
17. Rapid dissemination of taxonomic discoveries based on DNA barcoding and morphology
18. Genetic diversity in cytochrome c oxidase I gene of Anopheles mosquitoes
19. Services of DNA barcoding in different fields
20. Towards a DNA Barcode Reference Database for Spiders and Harvestmen of Germany
21. Spider cyberdiversity (Araneae: Araneomorphae) in an ecotouristic tropical forest fragment in Xilitla, Mexico
22. Determining threshold values for barcoding fungi: lessons from Cortinarius (Basidiomycota), a highly diverse and widespread ectomycorrhizal genus
23. DNA barcoding using skin exuviates can improve identification and biodiversity studies of snakes
24. DNA Barcoding of Medicinal Plants
25. Barcoding Neotropical birds: assessing the impact of nonmonophyly in a highly diverse group
26. DNA barcoding gap: reliable species identification over morphological and geographical scales
27. The Evolutionary History of the Rediscovered Austrian Population of the Giant Centipede Scolopendra cingulata Latreille 1829 (Chilopoda, Scolopendromorpha)
28. Developing DNA barcodes for species identification in Podophylloideae (Berberidaceae)
29. Does counting species count as taxonomy? On misrepresenting systematics, yet again
30. Testing the Potential of Proposed DNA Barcoding Markers in Nezara virudula and Nezara antennata When Geographic Variation and Closely Related Species Were Considered
31. DNA barcoding and taxonomic practice
32. Molecular Phylogeny, Evolution of Shell Shape, and DNA Barcoding in Polygyridae (Gastropoda: Pulmonata), an Endemic North American Clade of Land Snails
33. Testing mitochondrial marker efficacy for DNA barcoding in spiders: a test case using the dwarf spider genus Oedothorax (Araneae : Linyphiidae : Erigoninae)
34. Species-Specific Identification from Incomplete Sampling: Applying DNA Barcodes to Monitoring Invasive Solanum Plants
35. A critical review on the utility of DNA barcoding in biodiversity conservation
36. An emergent science on the brink of irrelevance: a review of the past 8 years of DNA barcoding
37. Phylogenetic Reconstruction and DNA Barcoding for Closely Related Pine Moth Species (Dendrolimus) in China with Multiple Gene Markers
38. A fuzzy‐set‐theory‐based approach to analyse species membership in DNA barcoding
39. A New Method for Species Identification via Protein-Coding and Non-Coding DNA Barcodes by Combining Machine Learning with Bioinformatic Methods
40. Cryptic Diversity of South African Trapdoor Spiders: Three New Species of Stasimopus Simon, 1892 (Mygalomorphae, Ctenizidae), and Redescription of Stasimopus robertsi Hewitt, 1910
41. DNA barcoding invasive insects: database roadblocks
42. An integrative morphological and molecular diagnostics for Typhlodromus pyri (Acari: Phytoseiidae)
43. Morphology to the rescue: molecular data and the signal of morphological characters in combined phylogenetic analyses-a case study from mysmenid spiders (Araneae, Mysmenidae), with comments on the evolution of web architecture
44. The study of fossil spider species
45. DNA barcoding: a taxonomic point of view
46. Complete DNA barcode reference library for a country's butterfly fauna reveals high performance for temperate Europe
47. Integrating DNA barcode data and taxonomic practice: Determination, discovery, and description
48. DNA barcoding: a six-question tour to improve users' awareness about the method
49. DNA barcoding and traditional taxonomy unified through Integrative Taxonomy: a view that challenges the debate questioning both methodologies
50. Barcoding Nature: Strategic Naturalization as Innovatory Practice in the Genomic Ordering of Things
51. Tyrannobdella rex N. Gen. N. Sp. and the Evolutionary Origins of Mucosal Leech Infestations
52. Estimating sample sizes for DNA barcoding
53. High level of endemism in Haiti's last remaining forests: a revision of Modisimus (Araneae: Pholcidae) on Hispaniola, using morphology and molecules
54. The Real maccoyii: Identifying Tuna Sushi with DNA Barcodes – Contrasting Characteristic Attributes and Genetic Distances
55. Testing taxonomic boundaries and the limit of DNA barcoding in the Siberian sturgeon, Acipenser baerii
56. Accelerated Species Inventory on Madagascar Using Coalescent-Based Models of Species Delineation
57. DNA barcoding will frequently fail in complicated groups: An example in wild potatoes
58. caos software for use in character‐based DNA barcoding
59. Exploring the utility of three plastid loci for biocoding the filmy ferns (Hymenophyllaceae) of Moorea
60. Beyond mtDNA: nuclear gene flow suggests taxonomic oversplitting in the little brown bat (Myotis lucifugus)
61. Systematics must Embrace Comparative Biology and Evolution, not Speed and Automation
62. Inferring Species Membership Using DNA Sequences with Back-Propagation Neural Networks
63. ‘Integrative taxonomy’ then and now: a response to Dayrat (2005)
64. Undisciplined thinking: morphology and Hennig’s unfinished revolution
65. Phenetic and DNA taxonomy; a comment on Waugh
66. DNA barcoding: the social frontier
67. Local scale DNA barcoding of bivalves (Mollusca): a case study
68. Spider mite (Acari: Tetranychidae) mitochondrial COI phylogeny reviewed: host plant relationships, phylogeography, reproductive parasites and barcoding
69. Yeasts Isolated from Neotropical Wood‐Boring Beetles in SE Peru
70. Recent advances in DNA taxonomy
71. A comparison of algorithms for the identification of specimens using DNA barcodes: examples from gymnosperms
72. The Philodromus pulchellus-group in the Mediterranean: taxonomic revision, phylogenetic analysis and biogeography (Araneae:Philodromidae)
73. The microarthropods of sub-Antarctic Prince Edward Island: a quantitative assessment
74. DNA Barcoding and Taxonomy in Diptera: A Tale of High Intraspecific Variability and Low Identification Success
75. Species Discovery versus Species Identification in DNA Barcoding Efforts: Response to Rubinoff
76. On the use of DNA sequences for determining the species limits of a polymorphic new species in the stink bug genus Halys (Heteroptera: Pentatomidae) from Pakistan
77. Molecular taxonomy in pholcid spiders (Pholcidae, Araneae): evaluation of species identification methods using CO1 and 16S rRNA
78. Identifying units for conservation using molecular systematics: the cautionary tale of the Karner blue butterfly
79. DNA Barcoding Applied to Invasive Leafminers (Diptera: Agromyzidae) in the Philippines
80. Are plant DNA barcodes a search for the Holy Grail?
81. DNA Barcoding: Error Rates Based on Comprehensive Sampling
82. On the application of molecular barcodes in toxinological research
83. The unholy trinity: taxonomy, species delimitation and DNA barcoding
84. Losing the plot: DNA “barcodes” and taxonomy
85. Reply to the comment by L. Prendini on "Identifying spiders through DNA barcodes"
86. Review: Inventing Home: Emigration, Gender and the Middle Class in Lebanon, 1870–1920
 Akram Fouad Khater

View Options

Get Access

Login options

Check if you access through your login credentials or your institution to get full access on this article.

Subscribe

Click on the button below to subscribe to Canadian Journal of Zoology

Purchase options

Purchase this article to get full access to it.

Restore your content access

Enter your email address to restore your content access:

Note: This functionality works only for purchases done as a guest. If you already have an account, log in to access the content to which you are entitled.

View options

PDF

View PDF

Media

Media

Other

Tables

Share Options

Share

Share the article link

Share on social media