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DNA barcoding and phylogenetic analysis of Pectinidae (Mollusca: Bivalvia) based on mitochondrial COI and 16S rRNA genes

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Abstract

DNA sequence data enable not only the inference of phylogenetic relationships but also provide an efficient method for species-level identifications under the terms DNA barcoding or DNA taxonomy. In this study, we have sequenced partial sequences of mitochondrial COI and 16S rRNA genes from 63 specimens of 8 species of Pectinidae to assess whether DNA barcodes can efficiently distinguish these species. Sequences from homologous regions of four other species of this family were gathered from GenBank. Comparisons of within and between species levels of sequence divergence showed that genetic variation between species exceeds variation within species. When using neighbour-joining clustering based on COI and 16S genes, all species fell into reciprocally monophyletic clades with high bootstrap values. These evidenced that these scallop species can be efficiently identified by DNA barcoding. Evolutionary relationships of Pectinidae were also examined using the two mitochondrial genes. The results are almost consistent with Waller’s classification, which was proposed on the basis of shell microstructure and the morphological characteristics of juveniles.

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Acknowledgments

We would like to thank to Dr. Jun Chen from Ocean University of China for providing the samples of Pectinidae. This study was supported by research grants from National High Technology Research and Development Program (2007AA09Z433), Chinese Ministry of Education (707041), and 973 Program (2010CB126406).

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  1. Fisheries College, Ocean University of China, 266003, Qingdao, China

    Yanwei Feng, Qi Li, Lingfeng Kong & Xiaodong Zheng

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  1. Yanwei Feng
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Correspondence to Qi Li.

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Feng, Y., Li, Q., Kong, L. et al. DNA barcoding and phylogenetic analysis of Pectinidae (Mollusca: Bivalvia) based on mitochondrial COI and 16S rRNA genes. Mol Biol Rep 38, 291–299 (2011). https://doi.org/10.1007/s11033-010-0107-1

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