Elsevier

Fitoterapia

Volume 74, Issues 7–8, December 2003, Pages 692-694
Fitoterapia

Short report
Antimicrobial activity of Andrographis paniculata

https://doi.org/10.1016/S0367-326X(03)00159-X Get rights and content

Abstract

The antimicrobial activity of aqueous extract, andrographolides and arabinogalactan proteins from Andrographis paniculata were evaluated. The aqueous extract showed significant antimicrobial activity, which may be due to the combined effect of the isolated arabinogalactan proteins and andrographolides.

Section snippets

Plant.

Andrographis paniculata Nees. (Acanthaceae), air-dried herb collected in September–October 2001 from the Campus of IIT, Kharagpur, West Bengal, India. A voucher specimen was deposited in the herbarium of the Vidyasagar University, Midnapore, West Bengal, India.

Uses in traditional medicine.

A. paniculata has been traditionally used as an anti-inflammatory [1], hepatoprotective [2], [3], antiviral [4], antioxidant [5], immune enhancement [6] herbal medicine.

Previously isolated classes of compounds.

Andrographolide, 14-deoxy-11-oxoandrographolide, 14-deoxy-11,12-didehydroandrographolide and neoandrographolide [7].

Tested materials.

Aqueous extract (AE), andrographolides (AND) fraction and arabinogalactan proteins (AGPs) fraction [8]. The AND fraction was obtained by extraction with 80% MeOH and chloroform extraction. The presence of andrographolide in the chloroform fraction was detected by TLC against reference-andrographolide (Sigma Chemical Co., USA) and quantification spectrophotometrical [9], [10] (yield: 0.3%). The AGPs fraction was obtained by extraction of the plant material with 80% MeOH and subsequent extraction

Studied activity.

Antibacterial and antifungal activity by antibiotic disc (HiMedia Laboratories Ltd., India) diffusion assay method [13].

Used microorganisms.

Listed in Table 1.

Results and conclusions.

Results obtained for antibacterial and antifungal activity of aqueous extracts, andrographolide and arabinogalactan proteins are reported in Table 1. The data revealed that there are significant antibacterial and antifungal activities of aqueous extract in comparison to some known antibiotics.

The present investigation reveals the biological value of the cumulative effects of AGPs and AND resulting in enhanced antimicrobial activities.

References (13)

  • Y.C. Shen et al.

    Planta Med

    (2000)
  • S.S. Handa et al.

    Indian J Med Res

    (1990)
  • S.S. Handa et al.

    Indian J Med Res

    (1990)
  • Holt S, Linda C. New Jersey: Carol Publishing Group,...
  • N.P. Trivedi et al.

    Indian J Exp Biol

    (2001)
  • C. Calabrese et al.

    J Phytother Res

    (2000)
There are more references available in the full text version of this article.

Cited by (0)

View full text