Novel peptides with HIV-1 reverse transcriptase inhibitory activity derived from the fruits of Quercus infectoria
Supaphorn Seetaha
Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand
Search for more papers by this authorSupa Hannongbua
Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand
Search for more papers by this authorCorresponding Author
Jatuporn Rattanasrisomporn
Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand
Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand
Correspondence
Kiattawee Choowongkomon, Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Email: [email protected]
Jatuporn Rattanasrisomporn, Department of Companion Animal Clinical Sciences, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand
Email: [email protected]
Search for more papers by this authorCorresponding Author
Kiattawee Choowongkomon
Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand
Omics Center for Agriculture, Bioresources, Food and Health, Kasetsart University, Bangkok, Thailand
Correspondence
Kiattawee Choowongkomon, Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Email: [email protected]
Jatuporn Rattanasrisomporn, Department of Companion Animal Clinical Sciences, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand
Email: [email protected]
Search for more papers by this authorSupaphorn Seetaha
Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand
Search for more papers by this authorSupa Hannongbua
Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand
Search for more papers by this authorCorresponding Author
Jatuporn Rattanasrisomporn
Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, Thailand
Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand
Correspondence
Kiattawee Choowongkomon, Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Email: [email protected]
Jatuporn Rattanasrisomporn, Department of Companion Animal Clinical Sciences, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand
Email: [email protected]
Search for more papers by this authorCorresponding Author
Kiattawee Choowongkomon
Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand
Omics Center for Agriculture, Bioresources, Food and Health, Kasetsart University, Bangkok, Thailand
Correspondence
Kiattawee Choowongkomon, Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Email: [email protected]
Jatuporn Rattanasrisomporn, Department of Companion Animal Clinical Sciences, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand
Email: [email protected]
Search for more papers by this authorAbstract
The HIV-1 reverse transcriptase (HIV-1 RT), which is responsible for transcription of viral RNA genomes into DNA genomes, has become an important target for the treatment of patients with HIV infection. Hydrolyzed peptides from plants are considered a new source of potential drugs. In order to develop new effective inhibitors, peptides extracted from 111 Asian medicinal plants were screened against the HIV-1 RT. The crude hydrolyzed peptides from the fruit peel of Quercus infectoria were selected for purification and peptide sequence determination by HPLC and LC-MS. Two peptides of interest were synthesized, and an IC50 test was performed to determine their ability to inhibit the HIV-1 RT. The IC50 values of the peptides AIHIILI and LIAVSTNIIFIVV were determined to be 274 ± 5.10 nm and 236.4 ± 7.07 nm, respectively. This indicated that these peptides could be further developed as potential HIV-1 RT inhibitors.
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
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