Abstract
Andrographolide, the active constituent isolated from the plant Andrographis paniculata, showed a significant dose dependent (0.75-12 mg/kg p.o. x 7) protective activity against paracetamol-induced toxicity on ex vivo preparation of isolated rat hepatocytes. It significantly increased the percent viability of the hepatocytes as tested by trypan blue exclusion and oxygen uptake tests. It completely antagonized the toxic effects of paracetamol on certain enzymes (GOT, GPT and alkaline phosphatase) in serum as well as in isolated hepatic cells. Andrographolide was found to be more potent than silymarin, a standard hepatoprotective agent.
MeSH terms
- Acetaminophen / antagonists & inhibitors*
- Acetaminophen / toxicity
- Alanine Transaminase / blood
- Alanine Transaminase / metabolism
- Alkaline Phosphatase / blood
- Alkaline Phosphatase / metabolism
- Animals
- Aspartate Aminotransferases / blood
- Aspartate Aminotransferases / metabolism
- Chemical and Drug Induced Liver Injury / enzymology
- Chemical and Drug Induced Liver Injury / pathology
- Chemical and Drug Induced Liver Injury / prevention & control*
- Diterpenes / pharmacology*
- Female
- Liver / drug effects
- Liver / enzymology
- Male
- Oxygen Consumption / drug effects
- Plants, Medicinal / chemistry*
- Rats
- Rats, Inbred Strains
- Silymarin / pharmacology
- Trypan Blue
Substances
- Diterpenes
- Silymarin
- Acetaminophen
- andrographolide
- Aspartate Aminotransferases
- Alanine Transaminase
- Alkaline Phosphatase
- Trypan Blue