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Effect of the atypical neuroleptic risperidone on morphology and S100B secretion in C6 astroglial lineage cells

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Abstract

We investigated the effect of the atypical neuroleptic risperidone on morphology and S100B secretion in C6 glioma cells, considering the putative involvement of astroglial cells in neuropsychiatric disorders. In the presence of high experimental doses of risperidone, C6 cells become stellate, with process-bearing cells and partial retraction of the cell body followed by detachment from the adhesion surface with practically no cell death. These results indicate that risperidone is able to interfere with C6 cell adhesion without toxic effects. RhoA activator LPA prevented the effects of risperidone on cell morphology. From 6 h risperidone induced a statistically significant increment of about 80% in S100B secretion. These data contribute to the proposal that glial cells are targets of risperidone, which could be involved in the therapeutic response of risperidone to improve autism symptoms.

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Acknowledgments

This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and FINEP/ Rede IBN 01.06.0842–00. We would like to thank Ms. Alessandra Heizelmann for technical support with cell culture.

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Authors and Affiliations

  1. Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600 anexo, Porto Alegre, RS, 90035-003, Brazil

    André Quincozes-Santos, Renata Torres Abib, Marina Concli Leite, Daiane Bobermin, Victorio Bambini-Junior, Carlos-Alberto Gonçalves & Carmem Gottfried

  2. Pervasive Developmental Disorders Program (ProTID), Unidade de Neurologia Pediátrica do Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Rudimar Riesgo & Carmem Gottfried

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  1. André Quincozes-Santos
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  2. Renata Torres Abib
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  3. Marina Concli Leite
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  4. Daiane Bobermin
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  5. Victorio Bambini-Junior
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  6. Carlos-Alberto Gonçalves
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  7. Rudimar Riesgo
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  8. Carmem Gottfried
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Correspondence to Carmem Gottfried.

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Quincozes-Santos, A., Abib, R.T., Leite, M.C. et al. Effect of the atypical neuroleptic risperidone on morphology and S100B secretion in C6 astroglial lineage cells. Mol Cell Biochem 314, 59–63 (2008). https://doi.org/10.1007/s11010-008-9765-x

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  • DOI: https://doi.org/10.1007/s11010-008-9765-x

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