The Genome Sequence of the SARS-Associated Coronavirus
Abstract
We sequenced the 29,751-base genome of the severe acute respiratory syndrome (SARS)–associated coronavirus known as the Tor2 isolate. The genome sequence reveals that this coronavirus is only moderately related to other known coronaviruses, including two human coronaviruses, HCoV-OC43 and HCoV-229E. Phylogenetic analysis of the predicted viral proteins indicates that the virus does not closely resemble any of the three previously known groups of coronaviruses. The genome sequence will aid in the diagnosis of SARS virus infection in humans and potential animal hosts (using polymerase chain reaction and immunological tests), in the development of antivirals (including neutralizing antibodies), and in the identification of putative epitopes for vaccine development.
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Abbreviations for amino acids: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
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We thank all the staff at the BCCA Genome Sciences Centre for helping to facilitate the rapid sequencing of the SARS-CoV genome; R. Tellier (Hospital for Sick Children) for information on primer sequences that amplify a 216–base pair region of the Pol gene; I. Sadowski (Department of Biochemistry and Molecular Biology) and J. Hobbs and his staff (Nucleic Acid and Protein Services Unit) of the University of British Columbia for rapid synthesis of PCR primers; F. Ouellette (University of British Columbia Bioinformatics Centre) for advice and assistance; the staff at the National Center for Biotechnology Information for rapidly processing and making available our sequence data; and anonymous reviewers for their useful suggestions. The BCCA Genome Sciences Centre is supported by the British Columbia Cancer Foundation, Genome Canada/Genome British Columbia, Western Economic Diversification, Canada Foundation for Innovation, British Columbia Knowledge Development Fund, Canadian Institutes of Health Research, Michael Smith Foundation for Health Research, and Natural Sciences and Engineering Research Council of Canada. Clones derived from the SARS virus are available from the Genome Sciences Centre (www.bcgsc.bc.ca).
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Science
Volume 300 | Issue 5624
30 May 2003
30 May 2003
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American Association for the Advancement of Science.
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Received: 19 April 2003
Accepted: 30 April 2003
Published in print: 30 May 2003
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