Direct Gene Transfer into Mouse Muscle in Vivo
Abstract
RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and β-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for β-galactosidase activity was localized to muscle cells following injection of the β-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.
Get full access to this article
View all available purchase options and get full access to this article.
References
ACSADI, G, unpublished data.
AUSBEL, F.M., CURRENT PROTOCOLS MO (1989).
BENVENISTY, N, DIRECT INTRODUCTION OF GENES INTO RATS AND EXPRESSION OF THE GENES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 9551 (1986).
DEWET, J.R., FIREFLY LUCIFERASE GENE - STRUCTURE AND EXPRESSION IN MAMMALIAN-CELLS, MOLECULAR AND CELLULAR BIOLOGY 7: 725 (1987).
DUBENSKY, T.W., DIRECT TRANSFECTION OF VIRAL AND PLASMID DNA INTO THE LIVER OR SPLEEN OF MICE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 7529 (1984).
EGLITIS, M.A., RETROVIRAL VECTORS FOR INTRODUCTION OF GENES INTO MAMMALIAN-CELLS, BIOTECHNIQUES 6: 608 (1988).
FELGNER, P.L., FOCUS 11: 21 (1989).
Felgner, P. L., Nature 337:387 (1989).
FELGNER, P.L., LIPOFECTION - A HIGHLY EFFICIENT, LIPID-MEDIATED DNA-TRANSFECTION PROCEDURE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 7413 (1987).
FINN, G.K., HOMOLOGOUS PLASMID RECOMBINATION IS ELEVATED IN IMMORTALLY TRANSFORMED-CELLS, MOLECULAR AND CELLULAR BIOLOGY 9: 4009 (1989).
FRANZINIARMSTRO.C, MYOLOGY: 125 (1986).
FRIEDMANN, T, PROGRESS TOWARD HUMAN-GENE THERAPY, SCIENCE 244: 1275 (1989).
GORMAN, C.M., RECOMBINANT GENOMES WHICH EXPRESS CHLORAMPHENICOL ACETYLTRANSFERASE IN MAMMALIAN-CELLS, MOLECULAR AND CELLULAR BIOLOGY 2: 1044 (1982).
Gorman, C. M., Proceedings of the National Academy of Sciences of the United States of America 79: 6777 (1982).
HIRT, B, SELECTIVE EXTRACTION OF POLYOMA DNA FROM INFECTED MOUSE CELL CULTURES, JOURNAL OF MOLECULAR BIOLOGY 26: 365 (1967).
KANEDA, Y, INCREASED EXPRESSION OF DNA COINTRODUCED WITH NUCLEAR-PROTEIN IN ADULT-RAT LIVER, SCIENCE 243: 375 (1989).
KRIEG, P.A., FUNCTIONAL MESSENGER-RNAS ARE PRODUCED BY SP6 INVITRO TRANSCRIPTION OF CLONED CDNAS, NUCLEIC ACIDS RESEARCH 12: 7057 (1984).
LAVITRANO, M, SPERM CELLS AS VECTORS FOR INTRODUCING FOREIGN DNA INTO EGGS - GENETIC-TRANSFORMATION OF MICE, CELL 57: 717 (1989).
LEDLEY, F.D., SOMATIC GENE-THERAPY FOR HUMAN-DISEASE - BACKGROUND AND PROSPECTS .1., JOURNAL OF PEDIATRICS 110: 1 (1987).
MALONE, R.W., FOCUS 11: 61 (1989).
MALONE, R.W., CATIONIC LIPOSOME-MEDIATED RNA TRANSFECTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 86: 6077 (1989).
MANNINO, R.J., LIPOSOME MEDIATED GENE-TRANSFER, BIOTECHNIQUES 6: 682 (1988).
Nicolau, C., Proceedings of the National Academy of Sciences of the United States of America 80: 1068 (1983).
NORTON, P.A., BACTERIAL BETA-GALACTOSIDASE AS A MARKER OF ROUS-SARCOMA VIRUS GENE-EXPRESSION AND REPLICATION, MOLECULAR AND CELLULAR BIOLOGY 5: 281 (1985).
PAUZA, C.D., PERSISTENT HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 INFECTION OF MONOBLASTOID CELLS LEADS TO ACCUMULATION OF SELF-INTEGRATED VIRAL-DNA AND TO PRODUCTION OF DEFECTIVE VIRIONS, JOURNAL OF VIROLOGY 63: 3700 (1989).
PRICE, J, LINEAGE ANALYSIS IN THE VERTEBRATE NERVOUS-SYSTEM BY RETROVIRUS-MEDIATED GENE-TRANSFER, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 156 (1987).
RALSTON, E, TRANSFER OF A PROTEIN ENCODED BY A SINGLE NUCLEUS TO NEARBY NUCLEI IN MULTINUCLEATED MYOTUBES, SCIENCE 244: 1066 (1989).
Reid, T. R., Journal of Biological Chemistry 264: 4583 (1989).
RUBIO, R, PENETRATION OF HORSERADISH-PEROXIDASE INTO TERMINAL CISTERNAE OF FROG SKELETAL-MUSCLE FIBERS AND BLOCKADE OF CAFFEINE CONTRACTURE BY CA++ DEPLETION, ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE 124: 57 (1972).
SEEGER, C, THE CLONED GENOME OF GROUND-SQUIRREL HEPATITIS-VIRUS IS INFECTIOUS IN THE ANIMAL, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 5849 (1984).
Shimohama, S., Molecular Brain Research 5: 271 (1989).
WU, G.Y., RECEPTOR-MEDIATED GENE DELIVERY AND EXPRESSION INVIVO, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 14621 (1988).
(0)eLetters
eLetters is a forum for ongoing peer review. eLetters are not edited, proofread, or indexed, but they are screened. eLetters should provide substantive and scholarly commentary on the article. Embedded figures cannot be submitted, and we discourage the use of figures within eLetters in general. If a figure is essential, please include a link to the figure within the text of the eLetter. Please read our Terms of Service before submitting an eLetter.
Log In to Submit a ResponseNo eLetters have been published for this article yet.
Information & Authors
Information
Published In
Science
Volume 247 | Issue 4949
23 March 1990
23 March 1990
Copyright
© 1990.
Submission history
Published in print: 23 March 1990
Authors
Metrics & Citations
Metrics
Article Usage
Altmetrics
Citations
Cite as
- Jon A. Wolff et al.
Export citation
Select the format you want to export the citation of this publication.
Cited by
- Can the Revolution in mRNA-Based Vaccine Technologies Solve the Intractable Health Issues of Current Ruminant Production Systems?, Vaccines, 12, 2, (152), (2024).https://doi.org/10.3390/vaccines12020152
- Advances in Poultry Vaccines: Leveraging Biotechnology for Improving Vaccine Development, Stability, and Delivery, Vaccines, 12, 2, (134), (2024).https://doi.org/10.3390/vaccines12020134
- Enhancing In Vivo Electroporation Efficiency through Hyaluronidase: Insights into Plasmid Distribution and Optimization Strategies, Pharmaceutics, 16, 4, (547), (2024).https://doi.org/10.3390/pharmaceutics16040547
- VEGF and Other Gene Therapies Improve Flap Survival—A Systematic Review and Meta-Analysis of Preclinical Studies, International Journal of Molecular Sciences, 25, 5, (2622), (2024).https://doi.org/10.3390/ijms25052622
- Solid Lipid Nanoparticles Delivering a DNA Vaccine Encoding Helicobacter pylori Urease A Subunit: Immune Analyses before and after a Mouse Model of Infection, International Journal of Molecular Sciences, 25, 2, (1076), (2024).https://doi.org/10.3390/ijms25021076
- Cholesterol-Bearing Polysaccharide-Based Nanogels for Development of Novel Immunotherapy and Regenerative Medicine, Gels, 10, 3, (206), (2024).https://doi.org/10.3390/gels10030206
- Electroporation in Translational Medicine: From Veterinary Experience to Human Oncology, Cancers, 16, 5, (1067), (2024).https://doi.org/10.3390/cancers16051067
- Recent Findings on Therapeutic Cancer Vaccines: An Updated Review, Biomolecules, 14, 4, (503), (2024).https://doi.org/10.3390/biom14040503
- Salmonella typhimurium Vaccine Candidate Delivering Infectious Bronchitis Virus S1 Protein to Induce Protection, Biomolecules, 14, 1, (133), (2024).https://doi.org/10.3390/biom14010133
- The next-generation DNA vaccine platforms and delivery systems: advances, challenges and prospects, Frontiers in Immunology, 15, (2024).https://doi.org/10.3389/fimmu.2024.1332939
- See more
Loading...
View Options
Check Access
Log in to view the full text
AAAS login provides access to Science for AAAS Members, and access to other journals in the Science family to users who have purchased individual subscriptions.
- Become a AAAS Member
- Activate your AAAS ID
- Purchase Access to Other Journals in the Science Family
- Account Help
Log in via OpenAthens.
Log in via Shibboleth.
More options
Purchase digital access to this article
Download and print this article for your personal scholarly, research, and educational use.
Buy a single issue of Science for just $15 USD.
View options
PDF format
Download this article as a PDF file
Download PDF