Abstract
A novel human Middle East respiratory syndrome coronavirus (MERS-CoV) caused outbreaks of severe acute respiratory syndrome (SARS)-like illness with a high mortality rate, raising concerns of its pandemic potential. Dipeptidyl peptidase-4 (DPP4) was recently identified as its receptor. Here we showed that residues 377 to 662 in the S protein of MERS-CoV specifically bound to DPP4-expressing cells and soluble DPP4 protein and induced significant neutralizing antibody responses, suggesting that this region contains the receptor-binding domain (RBD), which has a potential to be developed as a MERS-CoV vaccine.
Publication types
- Research Support, Non-U.S. Gov't
MeSH terms
- Animals
- Antibodies, Neutralizing / blood
- Antibodies, Viral / blood
- Binding Sites
- Cell Line
- Coronavirus / genetics
- Coronavirus / immunology
- Coronavirus / metabolism*
- Coronavirus Infections / virology
- Dipeptidyl Peptidase 4 / metabolism
- Humans
- Mice
- Mice, Inbred BALB C
- Protein Structure, Tertiary
- Receptors, Virus / metabolism
- Respiratory Tract Infections / virology
- Spike Glycoprotein, Coronavirus / genetics
- Spike Glycoprotein, Coronavirus / immunology
- Spike Glycoprotein, Coronavirus / metabolism*
- Viral Vaccines / genetics
- Viral Vaccines / immunology
Substances
- Antibodies, Neutralizing
- Antibodies, Viral
- Receptors, Virus
- Spike Glycoprotein, Coronavirus
- Viral Vaccines
- DPP4 protein, human
- Dipeptidyl Peptidase 4