Protection of K18-hACE2 mice and ferrets against SARS-CoV-2 challenge by a single-dose mucosal immunization with a parainfluenza virus 5–based COVID-19 vaccine
Abstract
INTRODUCTION
RESULTS
Generation and analysis of PIV5 expressing the S protein of SARS-CoV-2 (CVXGA1)
CVXGA1 generated humoral and cellular immune responses in mice
CVXGA1 protected mice with human ACE2 receptor against SARS-CoV-2 lethal challenge
CVXGA1 protected ferrets from SARS-CoV-2 infection
DISCUSSION
MATERIALS AND METHODS
Virus and cells
Immunofluorescence and immunoblotting
Purification of S and RBD of S
Enzyme-linked immunosorbent assay
Immunization and infection of mice with SARS-CoV-2
Immunization
Infection of mice
Immunization and infection of ferrets with SARS-CoV-2
Immunization of ferrets
Viral challenge of ferrets
Cohousing of ferrets
Histology and immunohistochemistry
SARS-CoV-2 S neutralization
SARS-CoV-2 nasal shedding qPCR
RNA extraction and generation of a standard curve
Ferret nasal wash qPCR
Ferret tissue qPCR
Focus-forming unit assay
Acknowledgments
Supplementary Material
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July 2021
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- Dong An et al.
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- A J Paramyxovirus-vectored HIV vaccine induces humoral and cellular responses in mice, Vaccine, (2024).https://doi.org/10.1016/j.vaccine.2024.02.068
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- Polymer nanoparticles deliver mRNA to the lung for mucosal vaccination, Science Translational Medicine, 15, 709, (2023)./doi/10.1126/scitranslmed.abq0603
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